Foot Fault Test
Objective: Assessment of motor deficits by measuring misplacement of forelimbs during locomotion on a grid
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
Induce transient or permanent middle cerebral artery occlusion
Perform either transient middle cerebral artery occlusion (tMCAO) or permanent distal MCAO (dMCAO) in wild-type and IL-4 knockout mice
Note: Two surgical approaches used in different animal groups
View evidence from paper
“Transient middle cerebral artery occlusion (tMCAO) or permanent distal MCAO (dMCAO) was induced in wild-type (WT) and IL-4 knockout (KO) C57/BL6 mice”
Administer IL-4 or vehicle infusion
In a separate cohort of WT animals, infuse IL-4 or vehicle into the cerebroventricle following tMCAO
Note: IL-4 dose: 60 ng/d. This is performed in WT animals only, after tMCAO induction
View evidence from paper
“In a separate cohort of WT animals, IL-4 (60 ng/d for 7d) or vehicle was infused into the cerebroventricle after tMCAO”
Perform foot fault test
Assess motor deficits by measuring misplacement of forelimbs during locomotion on a grid
Note: Part of a battery of behavioral tests including Rotarod, corner test, and Morris water maze
View evidence from paper
“Behavioral outcomes were assessed by the Rotarod, corner, foot fault, and Morris water maze tests”