Source Paper
Computational modeling reveals cognitive processes in simple rodent depression tests
Li Z, Lu T, Yan J, Zhang X, Li YF
Cell Rep Methods • 2025
Forced Swim Test (FST)
behavioralMouseC57Bl/6
Objective: Behavioral assessment of depression-like behavior through measurement of immobility time during forced swimming
Protocol Steps
1
Room preparation
Perform the test in a well-lit room
View evidence from paper
“The FST was performed in a well-lit room”
2
Water preparation
Fill cylinders with water at appropriate depth and temperature
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“at depth sufficient to prevent their limbs from touching the bottom”
3
Individual placement
Place each mouse individually in the water-filled cylinder
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“Mice were individually placed in water-filled cylinders”
4
Swimming session
Allow mouse to swim freely for 6 minutes
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“Each mouse was allowed to swim freely for 6 min”
5
Video recording
Record all movements during the swimming session
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“its movements were recorded on video”