Source Paper
Prenatal and early postnatal cannabis exposure interactions with adolescent chronic stress on anxiety-like, depression-like, and risk-taking behaviour.
Peterson CS, Ifionu I, Hamood F, Semizeh H, Ali A et al.
Psychopharmacology (Berl) • 2026
Forced Swim Test
behavioralmouse
Objective: Measures stress coping behavior in mice
Materials & Equipment Checklist
2 items1 from ConductScience
Gather these items before starting the experiment. Check off items as you prepare.
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Protocol Steps
1
Prenatal and postnatal THC exposure
Mouse dams orally consume THC in whole cannabis oil daily
From GD1-PD10
Note: Dose is 5 mg/kg daily
View evidence from paper
“Mouse dams orally consumed 5 mg/kg THC in whole cannabis oil daily from GD1-PD10”
2
Chronic mild unpredictable stress exposure
Offspring are exposed to chronic mild unpredictable stress throughout adolescence
PD28-56
View evidence from paper
“Offspring were exposed to chronic mild unpredictable stress throughout adolescence (PD28-56)”
3
Behavioral testing battery
Mice are challenged with a battery of tests including forced swim test to measure stress coping behavior
Note: Testing begins at PD58
View evidence from paper
“From PD58, mice were challenged with a battery of tests to measure anxiety-like (elevated plus maze, open field test), stress coping (forced swim test, tail suspension test)”
4
Brain slice collection
Brain slices are taken for c-Fos expression analysis
90 min after forced swim test
View evidence from paper
“Brain slices were taken 90 min after forced swim test to analyze c-Fos expression”