Source Paper
Electroacupuncture promotes BDNF-dependent neurogenesis via microglial reprogramming in a chronic stress model
Zhang L, Wei T, Liu X, Zhang L, Wang D et al.
Chin Med • 2026
Forced Swimming Test
behavioralMouseC57BL/6J
Objective: Assess the degree of desperation behaviors in an inescapable space
Materials & Equipment Checklist
3 items
Gather these items before starting the experiment. Check off items as you prepare.
Materials1
Not specified • Not specified
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Protocol Steps
1
Mouse placement
Place each mouse in transparent acrylic cylinder filled with water
Not specified
View evidence from paper
“Each mouse was filmed in a transparent acrylic cylinder (High: 25 cm, Depth: 10 cm) filled with 800 mL water”
2
Video recording
Film all trials using video camera
Not specified
View evidence from paper
“All trials were filmed by video camera (VisuTrack, shanghai, XR-VT)”
3
Behavioral scoring
Define immobility as minimal movement to keep head out of water, scored by competent blind observer
Not specified
View evidence from paper
“Immobility was defined as the animal making minimal movement to keep its head out of water, and a competent blind observer scored immobility”
4
Data analysis
Analyze total immobility time during the last 4 minutes of test
4 minutes
View evidence from paper
“The total immobility time during the last 4 min of test was the main indicator analyzed”