Source Paper
Electroacupuncture promotes BDNF-dependent neurogenesis via microglial reprogramming in a chronic stress model
Zhang L, Wei T, Liu X, Zhang L, Wang D et al.
Chin Med • 2026
Forced Swimming Test
Objective: Assess the degree of desperation behaviors in an inescapable space
This is a Forced Swimming Test protocol using Mouse as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 1 materials. Extracted from a 2026 paper published in Chin Med.
Model and subjects
Mouse • C57BL/6J • Male • 8-week-old • 44
Study window
~8 minutes hands-on
Core workflow
Mouse placement • Video recording • Behavioral scoring
Primary readouts
- Total immobility time during the last 4 minutes of test
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Mouse placement
Place each mouse in transparent acrylic cylinder filled with water
View evidence from paper
“Each mouse was filmed in a transparent acrylic cylinder (High: 25 cm, Depth: 10 cm) filled with 800 mL water”
Video recording
Film all trials using video camera
View evidence from paper
“All trials were filmed by video camera (VisuTrack, shanghai, XR-VT)”
Behavioral scoring
Define immobility as minimal movement to keep head out of water, scored by competent blind observer
View evidence from paper
“Immobility was defined as the animal making minimal movement to keep its head out of water, and a competent blind observer scored immobility”
Data analysis
Analyze total immobility time during the last 4 minutes of test
View evidence from paper
“The total immobility time during the last 4 min of test was the main indicator analyzed”