Grip Strength Test
Objective: Assessment of forelimb muscular strength in light and dark phases across multiple time points in zQ175 mice
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified in text • Not specified • Not specified • Not specified
Materials1
DataMars
1 item available from ConductScience
Estimated: $2,900.00
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Protocol Steps
Animal Identification
Mice were ear notched at 10-15 days of age for identification purposes
Note: Performed during early development
View evidence from paper
“Animals were ear notched at around 10–15 days”
RFID Chip Implantation
Electronic RFID chips were implanted for tracking purposes
Note: Performed at weaning
View evidence from paper
“Mice were weaned and implanted with RFID electronic chips (DataMars, OH) for identification at around 21 days”
Genotyping and CAG Repeat Determination
Tail snips were collected and sent for PCR analysis to determine genotype and CAG repeat count
Note: Performed by Laragen Inc. (Culver City, CA)
View evidence from paper
“Genotyping and CAG repeat count were determined by Laragen Inc. (Culver City, CA), from PCR of tail snips taken at 10–15 days of age”
Grip Strength Testing - Light Phase
Forelimb muscular strength assessment conducted during light phase
Note: Multiple timepoints across development and aging
View evidence from paper
“Grip Strength in the light phase tested at multiple ages as shown in Table 1”
Grip Strength Testing - Dark Phase
Forelimb muscular strength assessment conducted during dark phase
Note: Fewer timepoints than light phase testing
View evidence from paper
“Grip Strength in the dark phase tested at select timepoints as shown in Table 1”