Grip Strength Test
Objective: Assessment of forelimb muscular strength in light and dark phases across multiple time points in zQ175 mice
This is a Grip Strength Test protocol using mouse as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 1 materials. Extracted from a 2012 paper published in PLoS ONE.
Model and subjects
mouse • C57BL/6J background with zQ175 CAG repeat expansion • both • Tested at weeks 12, 13, 16, 17, 20, 21, 22, 24, 25, 28, 29, 30, 32, 33, 36, 37, 38, and 58 • 9-12 per sex per genotype in primary test cohort
Study window
~3 week study window
Core workflow
Animal Identification • RFID Chip Implantation • Genotyping and CAG Repeat Determination
Primary readouts
- Forelimb muscular strength in light phase
- Forelimb muscular strength in dark phase
- Comparison across genotypes (homozygous, heterozygous, wild type)
- Longitudinal changes across multiple timepoints from 12 to 58 weeks of age
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Animal Identification
Mice were ear notched at 10-15 days of age for identification purposes
Note: Performed during early development
View evidence from paper
“Animals were ear notched at around 10–15 days”
RFID Chip Implantation
Electronic RFID chips were implanted for tracking purposes
Note: Performed at weaning
View evidence from paper
“Mice were weaned and implanted with RFID electronic chips (DataMars, OH) for identification at around 21 days”
Genotyping and CAG Repeat Determination
Tail snips were collected and sent for PCR analysis to determine genotype and CAG repeat count
Note: Performed by Laragen Inc. (Culver City, CA)
View evidence from paper
“Genotyping and CAG repeat count were determined by Laragen Inc. (Culver City, CA), from PCR of tail snips taken at 10–15 days of age”
Grip Strength Testing - Light Phase
Forelimb muscular strength assessment conducted during light phase
Note: Multiple timepoints across development and aging
View evidence from paper
“Grip Strength in the light phase tested at multiple ages as shown in Table 1”
Grip Strength Testing - Dark Phase
Forelimb muscular strength assessment conducted during dark phase
Note: Fewer timepoints than light phase testing
View evidence from paper
“Grip Strength in the dark phase tested at select timepoints as shown in Table 1”