Source Paper
Fecal microbiota transplantation protects rotenone-induced Parkinson’s disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis
Zhe Zhao, Jingwen Ning, Xiu-qi Bao, Meiyu Shang, Jingwei Ma et al.
Microbiome • 2021
Grip Strength Test
Objective: Measurement of forelimb grip strength in mice
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Protocol Steps
Animal acclimation
Mice were acclimated under standard conditions with 12-hour light/dark cycle, temperature 22±2°C, humidity 50-60%, with ad libitum access to food and water
Note: Standard housing conditions prior to experimental procedures
View evidence from paper
“The mice were then acclimatized (12-h light/dark cycle) under standard conditions (temperature 22 ± 2 °C, humidity 50–60%) with ad libitum access to food and water for 7 days”
Group assignment
45 mice randomly assigned into control group (n=15) and model group (n=30)
Note: Random assignment to ensure unbiased group distribution
View evidence from paper
“A total of 45 mice were randomly assigned into two groups: the control group ( n = 15) and the model group ( n = 30)”
Initial treatment phase (weeks 1-4)
Model group received daily oral administration of rotenone; control group received vehicle administration
Note: Daily administration period to establish disease model
View evidence from paper
“In the beginning 4 weeks, the model group received the oral administration of rotenone every day. Meanwhile, the control group mice were administrated with vehicle”
Secondary group division (week 4)
Model group mice randomly divided into Rotenone group (n=15) and FMT group (n=15)
Note: Random division of model group for treatment comparison
View evidence from paper
“After 4 weeks, we randomly divided the model group mice into two groups: Rotenone group ( n = 15) and FMT group ( n = 15)”
Secondary treatment phase (weeks 5-6)
FMT group received FMT treatment once per day; control and Rotenone groups received vehicle administration once per day
Note: Treatment continuation with new intervention for FMT group
View evidence from paper
“During week 5 and 6, the mice in the FMT group were treated with FMT once per day. In the meantime, the control group and the rotenone group mice received vehicle administration once a day”
Body weight monitoring
All mice were weighed daily throughout the experimental period
Note: Daily measurement to track health status and treatment effects
View evidence from paper
“These mice were daily weighed for 6 weeks”
Grip strength test
Forelimb grip strength measurement performed at week 6
Note: Behavioral test conducted at endpoint (week 6) with n=15 per group
View evidence from paper
“behavioral tests were performed at week 6”
Animal sacrifice
All mice were sacrificed at week 6 for further analysis
Note: Terminal endpoint for tissue collection and analysis
View evidence from paper
“All the mice were sacrificed at week 6 for further analysis”