Experiments/Grip Strength Test

Source Paper

Fecal microbiota transplantation protects rotenone-induced Parkinson’s disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis

Zhe Zhao, Jingwen Ning, Xiu-qi Bao, Meiyu Shang, Jingwei Ma et al.

Microbiome • 2021

DOI PMC

Grip Strength Test

behavioralmouseC57BL/6J

Objective: Measurement of forelimb grip strength in mice

This is a Grip Strength Test protocol using mouse as the model organism. The procedure involves 8 procedural steps, 1 equipment items, 3 materials. Extracted from a 2021 paper published in Microbiome.

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Replication Essentials

Model and subjects

mouse • C57BL/6J • male • 8 weeks • 20-22 g • 45

Study window

~6 week study window

Core workflow

Animal acclimation • Group assignment • Initial treatment phase (weeks 1-4)

Primary readouts

Forelimb grip strength values

Key equipment and reagents

Grip strength testing apparatusRotenoneVehicle control

Before You Start

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Protocol Steps

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Animal acclimation

Mice were acclimated under standard conditions with 12-hour light/dark cycle, temperature 22±2°C, humidity 50-60%, with ad libitum access to food and water

7 days22 ± 2°C

Note: Standard housing conditions prior to experimental procedures

View evidence from paper

“The mice were then acclimatized (12-h light/dark cycle) under standard conditions (temperature 22 ± 2 °C, humidity 50–60%) with ad libitum access to food and water for 7 days”

Group assignment

45 mice randomly assigned into control group (n=15) and model group (n=30)

Note: Random assignment to ensure unbiased group distribution

View evidence from paper

“A total of 45 mice were randomly assigned into two groups: the control group ( n = 15) and the model group ( n = 30)”

Initial treatment phase (weeks 1-4)

Model group received daily oral administration of rotenone; control group received vehicle administration

4 weeks

Note: Daily administration period to establish disease model

View evidence from paper

“In the beginning 4 weeks, the model group received the oral administration of rotenone every day. Meanwhile, the control group mice were administrated with vehicle”

Secondary group division (week 4)

Model group mice randomly divided into Rotenone group (n=15) and FMT group (n=15)

Note: Random division of model group for treatment comparison

View evidence from paper

“After 4 weeks, we randomly divided the model group mice into two groups: Rotenone group ( n = 15) and FMT group ( n = 15)”

Secondary treatment phase (weeks 5-6)

FMT group received FMT treatment once per day; control and Rotenone groups received vehicle administration once per day

2 weeks

Note: Treatment continuation with new intervention for FMT group

View evidence from paper

“During week 5 and 6, the mice in the FMT group were treated with FMT once per day. In the meantime, the control group and the rotenone group mice received vehicle administration once a day”

Body weight monitoring

All mice were weighed daily throughout the experimental period

6 weeks

Note: Daily measurement to track health status and treatment effects

View evidence from paper

“These mice were daily weighed for 6 weeks”