H. polygyrus bakeri Infection

“Mice were infected orally by gavage with 200 infective L3 stage larvae”

“mice were injected i.p. with tetrahydrolipistatin (Orlistat; 200 mg/kg, Cayman Chemical)”

“300 μl of 3% thioglycollate (Sigma) immediately prior to IL-4 complexed to mAb anti-IL-4”

“IL-4c; containing 5 μg of IL-4, PeproTech, and 25 μg of anti-IL-4 clone 11B11, BioXcell”

“adult H. polygyrus were eliminated from infected mice by oral administration of pyrantel pamoate (1 mg/mouse; Columbia Laboratory) at day 14 post-primary infection”

“> 5 weeks later the mice were challenge-infected with 200 L3 stage larvae by gavage”

“At day 9 post challenge infection, mice were sacrificed and parasite burdens were measured”

“intestines were removed, opened longitudinally, and placed into a metal strainer on top of a 50 ml tube filled with phosphate buffered saline (PBS; Corning Inc.) for 4 h at 37 °C. Parasites dropped through the filter into the tube and were recovered for counting on a dissecting microscope”

“Parasite eggs were enumerated by floating eggs in feces collected from individual mice on saturated sodium chloride prior to collection, and counting under a microscope”