HDAC Inhibitor Infusion and Behavioral Testing
Objective: To assess antidepressant-like effects of HDAC inhibitor MS-275 infusion into the nucleus accumbens using behavioral assays
This is a HDAC Inhibitor Infusion and Behavioral Testing protocol using mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 2 materials. Extracted from a 2009 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Chronic social defeat stress exposure • HDAC inhibitor infusion into nucleus accumbens • Behavioral assays
Primary readouts
- Antidepressant-like behavioral effects in social defeat paradigm
- Performance in other behavioral assays
- Global patterns of gene expression in nucleus accumbens
- Histone H3 acetylation levels
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Chronic social defeat stress exposure
Subjects exposed to chronic social defeat stress paradigm prior to HDAC inhibitor infusion
Note: This stress paradigm produces persistent increase in H3 acetylation in nucleus accumbens
View evidence from paper
“chronic social defeat stress in mice causes a transient decrease, followed by a persistent increase, in levels of acetylated histone H3 in the nucleus accumbens”
HDAC inhibitor infusion into nucleus accumbens
Infusion of MS-275 HDAC inhibitor directly into the nucleus accumbens to increase histone acetylation
Note: Infusion increases histone acetylation and produces antidepressant-like effects
View evidence from paper
“infusion of HDAC inhibitors into the nucleus accumbens, which increases histone acetylation, exerts robust antidepressant-like effects in the social defeat paradigm and other behavioral assays”
Behavioral assays
Conduct behavioral testing to assess antidepressant-like effects following HDAC inhibitor infusion
Note: Multiple behavioral assays used to measure antidepressant-like effects
View evidence from paper
“exerts robust antidepressant-like effects in the social defeat paradigm and other behavioral assays”
Gene expression analysis
Collect nucleus accumbens tissue and perform microarray analysis to determine global patterns of gene expression
Note: Results compared to effects of fluoxetine treatment
View evidence from paper
“HDAC inhibitor infusion also reverses the effects of chronic defeat stress on global patterns of gene expression in the nucleus accumbens, as determined by microarray analysis”