Healthspan Assessment
Objective: Evaluation of healthspan and functional outcomes in mice with perinatal, post-pubertal, and late-adult IGF-1 deficiency to determine how alterations in IGF-1 levels at multiple stages influence overall lifespan, healthspan, and pathology
This is a Healthspan Assessment protocol using mouse as the model organism. The procedure involves 7 procedural steps. Extracted from a 2017 paper published in GeroScience.
Model and subjects
mouse • igf f/f C57Bl/6 • both • Multiple timepoints: perinatal, post-pubertal, and late-adult
Study window
Estimated timing pending
Core workflow
IGF-1 Deficiency Induction - Perinatal • IGF-1 Deficiency Induction - Post-pubertal • IGF-1 Deficiency Induction - Late-adult
Primary readouts
- Lifespan
- Healthspan
- Pathology
- Cancer incidence and risk
Key equipment and reagents
Verified items
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Direct vendor links
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Protocol Steps
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IGF-1 Deficiency Induction - Perinatal
Induce IGF-1 deficiency during perinatal period using genetic approach in igf f/f C57Bl/6 mice
Note: Perinatal timing represents early developmental stage
View evidence from paper
“investigated the effects of perinatal, post-pubertal, and late-adult onset IGF-1 deficiency using genetic and viral approaches”
IGF-1 Deficiency Induction - Post-pubertal
Induce IGF-1 deficiency during post-pubertal period using genetic and/or viral approaches in igf f/f C57Bl/6 mice
Note: Post-pubertal timing represents adolescent/young adult stage
View evidence from paper
“investigated the effects of perinatal, post-pubertal, and late-adult onset IGF-1 deficiency using genetic and viral approaches”
IGF-1 Deficiency Induction - Late-adult
Induce IGF-1 deficiency during late-adult period using genetic and/or viral approaches in igf f/f C57Bl/6 mice
Note: Late-adult timing is relevant for human studies and represents aged animals
View evidence from paper
“late-life IGF-1 deficiency (a time point relevant for human studies) reduces cancer risk but does not increase lifespan”
Assess Lifespan
Monitor and record lifespan outcomes across all treatment groups and timepoints
Note: Measured in both male and female mice
View evidence from paper
“examined how alterations in IGF-1 levels at multiple stages of development and aging influence overall lifespan, healthspan, and pathology”
Assess Healthspan
Evaluate healthspan and functional outcomes in mice across all treatment groups and developmental timepoints
Note: Healthspan assessment is sex-specific and tissue-specific
View evidence from paper
“examined how alterations in IGF-1 levels at multiple stages of development and aging influence overall lifespan, healthspan, and pathology”
Assess Pathology
Evaluate pathological changes and tissue health across all treatment groups and developmental timepoints
Note: Changes are specific for each sex and tissue
View evidence from paper
“these changes are specific for each sex and tissue”
Cancer Risk Assessment
Monitor and assess cancer incidence and risk across all treatment groups
Note: Late-life IGF-1 deficiency reduces cancer risk
View evidence from paper
“late-life IGF-1 deficiency (a time point relevant for human studies) reduces cancer risk but does not increase lifespan”