IGF-1 has sexually dimorphic, pleiotropic, and time-dependent effects on healthspan, pathology, and lifespan

Nicole M. Ashpole, Sreemathi Logan, Andriy Yabluchanskiy, Matthew C. Mitschelen, Han Yan et al.

GeroScience • 2017

DOI PMC

Healthspan Assessment

behavioralmouseigf f/f C57Bl/6

Objective: Evaluation of healthspan and functional outcomes in mice with perinatal, post-pubertal, and late-adult IGF-1 deficiency to determine how alterations in IGF-1 levels at multiple stages influence overall lifespan, healthspan, and pathology

Protocol Steps

IGF-1 Deficiency Induction - Perinatal

Induce IGF-1 deficiency during perinatal period using genetic approach in igf f/f C57Bl/6 mice

Note: Perinatal timing represents early developmental stage

View evidence from paper

“investigated the effects of perinatal, post-pubertal, and late-adult onset IGF-1 deficiency using genetic and viral approaches”

IGF-1 Deficiency Induction - Post-pubertal

Induce IGF-1 deficiency during post-pubertal period using genetic and/or viral approaches in igf f/f C57Bl/6 mice

Note: Post-pubertal timing represents adolescent/young adult stage

View evidence from paper

“investigated the effects of perinatal, post-pubertal, and late-adult onset IGF-1 deficiency using genetic and viral approaches”

IGF-1 Deficiency Induction - Late-adult

Induce IGF-1 deficiency during late-adult period using genetic and/or viral approaches in igf f/f C57Bl/6 mice

Note: Late-adult timing is relevant for human studies and represents aged animals

View evidence from paper

“late-life IGF-1 deficiency (a time point relevant for human studies) reduces cancer risk but does not increase lifespan”

Assess Lifespan

Monitor and record lifespan outcomes across all treatment groups and timepoints

Throughout entire lifespan

Note: Measured in both male and female mice

View evidence from paper

“examined how alterations in IGF-1 levels at multiple stages of development and aging influence overall lifespan, healthspan, and pathology”

Assess Healthspan

Evaluate healthspan and functional outcomes in mice across all treatment groups and developmental timepoints

Throughout lifespan at multiple timepoints

Note: Healthspan assessment is sex-specific and tissue-specific

View evidence from paper

“examined how alterations in IGF-1 levels at multiple stages of development and aging influence overall lifespan, healthspan, and pathology”

Assess Pathology

Evaluate pathological changes and tissue health across all treatment groups and developmental timepoints

Throughout lifespan at multiple timepoints

Note: Changes are specific for each sex and tissue

View evidence from paper

“these changes are specific for each sex and tissue”

Cancer Risk Assessment

Monitor and assess cancer incidence and risk across all treatment groups

Throughout lifespan

Note: Late-life IGF-1 deficiency reduces cancer risk

View evidence from paper

“late-life IGF-1 deficiency (a time point relevant for human studies) reduces cancer risk but does not increase lifespan”