Mice with Deficient BK Channel Function Show Impaired Prepulse Inhibition and Spatial Learning, but Normal Working and Spatial Reference Memory

Marei Typlt, Magdalena Mirkowski, Erin Azzopardi, Lukas Ruettiger, Peter Ruth et al.

PLoS ONE • 2013

DOI PMC

Hearing Test

behavioralmouseF1 generation hybrid SV129/C57BL6 line with deficient BK channel function

Objective: Assessment of hearing function in mice with altered BK channel function

Materials & Equipment Checklist

1 item

Gather these items before starting the experiment. Check off items as you prepare.

Materials1

Ear tags for individual mouse identification

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Protocol Steps

Mouse breeding and genotyping

Heterozygous C57BL6 mice were paired with heterozygous SV129 mice to produce F1 generation hybrid offspring with deficient BK channel function. Mice were generated and genotyped at the Pharmaceutical Institute, University of Tübingen, Germany.

Note: Exclusively F1 generation mice were tested to avoid effects of inbreeding. Each mouse received an individual ear-tag when collecting DNA sample for genotyping.

View evidence from paper

“Heterozygous C57BL6 mice were paired with heterozygous SV129 mice. All mice were generated and genotyped at the Pharmaceutical Institute, University of Tübingen, Germany. In order to ensure proper tracking each mouse got an individual ear-tag when collecting the DNA sample for the genotyping.”

Mouse transportation and acclimation

Mice were shipped to Canada at the age of 1.5-3 months and subsequently allowed to acclimate for 2 weeks before behavioral testing started.

2 weeks acclimation period

Note: Mice were shipped from University of Tübingen, Germany to Canada

View evidence from paper

“The mice were shipped to Canada at the age of 1.5-3 months and subsequently allowed to acclimate for 2 weeks before behavioural testing started.”

Housing conditions during testing period

Mice were group housed with mixed genetic background within groups, maintained on a 12 hour light-dark cycle with ad libitum food and water access.

Note: Testing only occurred during the light cycle from 7am to 7pm. If animals were tested repeatedly over several days, the time of testing for each animal was kept constant.

View evidence from paper

“Mice were group housed with mixed genetic background within groups, with a 12 hour light-dark cycle and with ad libitum food and water. Testing only occurred during the light cycle from 7am to 7pm. If animals were tested repeatedly over several days, the time of testing for each animal was kept constant.”

Hearing test performance

The hearing test was performed on a separate set of animals. Specific details of the hearing test procedure are referenced in respective method sections but not detailed in this excerpt.

Note: Different randomly selected subsets of the 56 total animals were used for the hearing test and other single tests.

View evidence from paper

“The hearing test was performed on a separate set of animals (for details see respective method sections).”