Source Paper
Ly-6Chi monocytes dominate hypercholesterolemia-associated monocytosis and give rise to macrophages in atheromata
Filip K. Swirski, Peter Libby, Elena Aikawa, Pilar Alcaide, F. William Luscinskas et al.
Journal of Clinical Investigation • 2007
High-Fat Diet Atherosclerosis Model
Objective: To investigate the role of Ly-6C hi monocytes in hypercholesterolemia-associated monocytosis and atherosclerosis development in apoE-deficient mice consuming a high-fat diet
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Materials1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
High-fat diet administration
apoE-deficient mice were fed a high-fat diet to induce hypercholesterolemia and atherosclerosis
Note: Mice were monitored for development of hypercholesterolemia-associated monocytosis (HAM)
View evidence from paper
“hypercholesterolemic apoE–deficient mice consuming a high-fat diet, with the number of Ly-6C hi cells doubling in the blood every month”
Monocyte monitoring and characterization
Circulating monocytes were monitored for Ly-6C hi and Ly-6C lo subsets; Ly-6C hi monocytes were tracked for adhesion to activated endothelium and infiltration into lesions
Note: Ly-6C hi monocytes were observed to adhere to activated endothelium, infiltrate lesions, and become lesional macrophages
View evidence from paper
“Ly-6C hi monocytes adhered to activated endothelium, infiltrated lesions, and became lesional macrophages”
Statin treatment (optional endpoint)
Statin-induced cholesterol reduction was used to assess whether HAM subsided upon cholesterol reduction
Note: This was used as a control condition to demonstrate reversibility of the monocytosis
View evidence from paper
“subsided upon statin-induced cholesterol reduction”