High-Fat Diet Study in Fxr ΔIE Mice
Objective: Measurement of diet-induced obesity and weight gain in intestine-specific Fxr-null (Fxr ΔIE) mice fed high-fat diet, and comparison with tempol-treated wild-type mice to determine the role of intestinal FXR in obesity regulation
This is a High-Fat Diet Study in Fxr ΔIE Mice protocol using mouse as the model organism. The procedure involves 5 procedural steps, 2 materials. Extracted from a 2013 paper published in Nature Communications.
Model and subjects
mouse • Fxr ΔIE (intestine-specific Fxr-null) and wild-type controls • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Animal grouping and diet assignment • High-fat diet feeding • Tempol treatment (wild-type control group)
Primary readouts
- Diet-induced weight gain
- Obesity development
- Comparison of weight gain between Fxr ΔIE mice and wild-type controls
- Response to tempol treatment in both genotypes
Key equipment and reagents
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Protocol Steps
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Animal grouping and diet assignment
Intestine-specific Fxr-null (Fxr ΔIE) mice and wild-type control mice were assigned to high-fat diet feeding
Note: Study includes comparison between Fxr ΔIE mice and tempol-treated wild-type mice
View evidence from paper
“High-fat diet-fed intestine-specific Fxr-null (Fxr ΔIE) mice show lower diet-induced obesity, similar to tempol-treated wild-type mice”
High-fat diet feeding
Mice were fed high-fat diet to induce obesity and measure diet-induced weight gain
Note: Weight gain and obesity development were measured as primary outcomes
View evidence from paper
“High-fat diet-fed intestine-specific Fxr-null (Fxr ΔIE) mice show lower diet-induced obesity”
Tempol treatment (wild-type control group)
Wild-type mice received tempol treatment to alter gut microbiome composition and reduce Lactobacillus genus and bile salt hydrolase activity
Note: Tempol treatment leads to preferential reduction of Lactobacillus and accumulation of intestinal tauro-β-muricholic acid (T-β-MCA)
View evidence from paper
“tempol alters the gut microbiome by preferentially reducing the genus Lactobacillus and its bile salt hydrolase (BSH) activity”
Weight measurement and obesity assessment
Diet-induced weight gain and obesity development were measured in both Fxr ΔIE mice and tempol-treated wild-type mice
Note: Fxr ΔIE mice showed lower diet-induced obesity similar to tempol-treated wild-type mice
View evidence from paper
“High-fat diet-fed intestine-specific Fxr-null (Fxr ΔIE) mice show lower diet-induced obesity, similar to tempol-treated wild-type mice”
Tempol treatment validation in Fxr ΔIE mice
Tempol treatment was applied to Fxr ΔIE mice to determine if intestinal FXR mediates the anti-obesity effects
Note: Tempol treatment did not decrease weight gain in Fxr ΔIE mice, demonstrating that intestinal FXR is required for tempol's anti-obesity effects
View evidence from paper
“tempol treatment does not decrease weight gain in Fxr ΔIE mice, suggesting that the intestinal FXR mediates the anti-obesity effects of tempol”