High-Fat Diet Study in Fxr ΔIE Mice
Objective: Measurement of diet-induced obesity and weight gain in intestine-specific Fxr-null (Fxr ΔIE) mice fed high-fat diet, and comparison with tempol-treated wild-type mice to determine the role of intestinal FXR in obesity regulation
Gather these items before starting the experiment. Check off items as you prepare.
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Animal grouping and diet assignment
Intestine-specific Fxr-null (Fxr ΔIE) mice and wild-type control mice were assigned to high-fat diet feeding
Note: Study includes comparison between Fxr ΔIE mice and tempol-treated wild-type mice
View evidence from paper
“High-fat diet-fed intestine-specific Fxr-null (Fxr ΔIE) mice show lower diet-induced obesity, similar to tempol-treated wild-type mice”
High-fat diet feeding
Mice were fed high-fat diet to induce obesity and measure diet-induced weight gain
Note: Weight gain and obesity development were measured as primary outcomes
View evidence from paper
“High-fat diet-fed intestine-specific Fxr-null (Fxr ΔIE) mice show lower diet-induced obesity”
Tempol treatment (wild-type control group)
Wild-type mice received tempol treatment to alter gut microbiome composition and reduce Lactobacillus genus and bile salt hydrolase activity
Note: Tempol treatment leads to preferential reduction of Lactobacillus and accumulation of intestinal tauro-β-muricholic acid (T-β-MCA)
View evidence from paper
“tempol alters the gut microbiome by preferentially reducing the genus Lactobacillus and its bile salt hydrolase (BSH) activity”
Weight measurement and obesity assessment
Diet-induced weight gain and obesity development were measured in both Fxr ΔIE mice and tempol-treated wild-type mice
Note: Fxr ΔIE mice showed lower diet-induced obesity similar to tempol-treated wild-type mice
View evidence from paper
“High-fat diet-fed intestine-specific Fxr-null (Fxr ΔIE) mice show lower diet-induced obesity, similar to tempol-treated wild-type mice”
Tempol treatment validation in Fxr ΔIE mice
Tempol treatment was applied to Fxr ΔIE mice to determine if intestinal FXR mediates the anti-obesity effects
Note: Tempol treatment did not decrease weight gain in Fxr ΔIE mice, demonstrating that intestinal FXR is required for tempol's anti-obesity effects
View evidence from paper
“tempol treatment does not decrease weight gain in Fxr ΔIE mice, suggesting that the intestinal FXR mediates the anti-obesity effects of tempol”