High-Fat Diet Intake Measurement
Objective: Quantification of high-fat diet consumption in mice under chronic social defeat stress conditions, assessing stress-induced food-reward behavior
This is a High-Fat Diet Intake Measurement protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 2011 paper published in Journal of Clinical Investigation.
Model and subjects
mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Chronic Social Defeat Stress (CSDS) Exposure • Conditioned Place Preference Task • High-Fat Diet Intake Measurement
Primary readouts
- Conditioned place preference (CPP) for high-fat diet
- High-fat diet intake quantity
- Stress-induced food-reward behavior
- Ghrelin signaling dependency on food-reward behavior
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Chronic Social Defeat Stress (CSDS) Exposure
Mice are exposed to chronic social defeat stress, a model of prolonged psychosocial stress featuring aspects of major depression and posttraumatic stress disorder
Note: CSDS is the stressor condition under which food-reward behavior is subsequently measured
View evidence from paper
“exposure to chronic social defeat stress (CSDS), a model of prolonged psychosocial stress, featuring aspects of major depression and posttraumatic stress disorder”
Conditioned Place Preference Task
Food-reward behavior is assessed using a CPP task in mice following CSDS exposure
Note: CPP measures preference for environments associated with high-fat diet consumption
View evidence from paper
“food-reward behavior, assessed using a conditioned place preference (CPP) task, is monitored in mice after exposure to chronic social defeat stress”
High-Fat Diet Intake Measurement
Quantification of high-fat diet consumption is measured in stressed mice
Note: CSDS increased both CPP for and intake of high-fat diet
View evidence from paper
“CSDS increased both CPP for and intake of high-fat diet, and stress-induced food-reward behavior was dependent on signaling by the peptide hormone ghrelin”