Source Paper
Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age
Hirotaka Shoji, Keizo Takao, Satoko Hattori, Tsuyoshi Miyakawa
Molecular Brain • 2016
Hot Plate Test
Objective: Assessment of pain sensitivity and nociceptive response in mice
This is a Hot Plate Test protocol using mouse as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 4 materials. Extracted from a 2016 paper published in Molecular Brain.
Model and subjects
mouse • C57BL/6J and genetically engineered mice with C57BL/6J genetic background • male • Not specified • Not specified • 1739
Study window
~1 day study window
Core workflow
Animal housing and maintenance • Test scheduling and timing • Hot plate test administration
Primary readouts
- Pain sensitivity response
- Nociceptive response latency
Key equipment and reagents
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Protocol Steps
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Animal housing and maintenance
Mice housed in plastic cages with sterilized PaperClean Bedding under 12-hour light/dark cycle with lights on at 7:00 a.m. Food (CRF-1) and water provided ad libitum.
Note: Housing density varied: 1.3% with 1 animal, 2.3% with 2 animals, 6.7% with 3 animals, 80.1% with 4 animals, 8.9% with 5 animals, and 0.7% with 6 animals per cage at beginning of test battery
View evidence from paper
“housed in plastic cages with sterilized PaperClean Bedding (Japan SLC) under a 12-hr light/dark cycle (lights on at 7:00 a.m.) with access to food (CRF-1, Oriental Yeast Co., Ltd.) and water ad libitum”
Test scheduling and timing
Hot plate test performed as part of behavioral test battery. Behavioral testing conducted between 9:00 a.m. and 6:00 p.m. with at least 1 day interval between tests.
Note: Hot plate test was performed after light/dark transition, open field, and elevated plus maze tests, and before social interaction test. More than 75% of mice tested in accordance with standard test battery order.
View evidence from paper
“Behavioral testing was performed between 9:00 a.m. and 6:00 p.m. The interval between tests was at least 1 day.”
Hot plate test administration
Mice subjected to hot plate test to assess pain sensitivity and nociceptive response
Note: Specific parameters of hot plate test (temperature, latency measurement, cutoff time) not detailed in provided text
View evidence from paper
“hot plate”
Apparatus cleaning
All apparatus cleaned with super hypochlorous water and 70% ethanol after testing to prevent bias due to olfactory cues
Note: Cleaning performed between tests to eliminate olfactory cues
View evidence from paper
“After the tests, all apparatus were cleaned with super hypochlorous water and 70% ethanol to prevent a bias due to olfactory cues.”