Housing Condition Study
behavioralmouseC57BL/6N
Objective: Comparison of grouped versus isolated housing conditions on mice with SNI or sham surgery to assess effects on behavioral outcomes
Protocol Steps
1
Animal acquisition and delivery
C57BL/6N male mice were purchased from Charles River Laboratories at 8 weeks of age
Note: Mice sourced from Sulzfeld, Germany
View evidence from paper
“C57BL/6N male mice were purchased from Charles River Laboratories (Sulzfeld, Germany) at the age of eight weeks”
2
Housing condition assignment
Immediately after delivery, mice were divided into two different housing conditions: grouped housing (3 mice per cage) or isolated housing (individual cages)
Immediately after delivery
Note: Either SNI or sham animals were caged together in grouped condition
View evidence from paper
“Immediately after delivery, mice were divided into two different housing conditions. For the grouped-housed condition, mice were housed in groups of three per cage. Either SNI or sham animals were caged together. For the isolated-housing condition animals were housed individually”
3
Environmental condition maintenance
All animals maintained under standard housing conditions with food and water ad libitum, 12-hour light/dark cycle (lights on 7 a.m.–7 p.m.), regulated ambient temperature of ±22°C, and relative humidity of 40%–50%
Throughout housing period±22°C
Note: Humidity maintained at 40%–50%
View evidence from paper
“All animals were housed with food and water ad libitum under a standard 12-h light/dark cycle (light on between 7 a.m.–7 p.m.) with regulated ambient temperature of ± 22°C and at relative humidity of 40%–50%”