Housing Condition Study
Objective: Comparison of grouped versus isolated housing conditions on mice with SNI or sham surgery to assess effects on behavioral outcomes
This is a Housing Condition Study protocol using mouse as the model organism. The procedure involves 3 procedural steps, 5 equipment items, 1 materials. Extracted from a 2016 paper published in Molecular Pain.
Model and subjects
mouse • C57BL/6N • male • 8 weeks
Study window
~8 week study window
Core workflow
Animal acquisition and delivery • Housing condition assignment • Environmental condition maintenance
Primary readouts
- Effects of housing condition (grouped vs. isolated) on behavioral outcomes in SNI and sham surgery mice
Key equipment and reagents
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Protocol Steps
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Animal acquisition and delivery
C57BL/6N male mice were purchased from Charles River Laboratories at 8 weeks of age
Note: Mice sourced from Sulzfeld, Germany
View evidence from paper
“C57BL/6N male mice were purchased from Charles River Laboratories (Sulzfeld, Germany) at the age of eight weeks”
Housing condition assignment
Immediately after delivery, mice were divided into two different housing conditions: grouped housing (3 mice per cage) or isolated housing (individual cages)
Note: Either SNI or sham animals were caged together in grouped condition
View evidence from paper
“Immediately after delivery, mice were divided into two different housing conditions. For the grouped-housed condition, mice were housed in groups of three per cage. Either SNI or sham animals were caged together. For the isolated-housing condition animals were housed individually”
Environmental condition maintenance
All animals maintained under standard housing conditions with food and water ad libitum, 12-hour light/dark cycle (lights on 7 a.m.–7 p.m.), regulated ambient temperature of ±22°C, and relative humidity of 40%–50%
Note: Humidity maintained at 40%–50%
View evidence from paper
“All animals were housed with food and water ad libitum under a standard 12-h light/dark cycle (light on between 7 a.m.–7 p.m.) with regulated ambient temperature of ± 22°C and at relative humidity of 40%–50%”