IL-6 Knockout Mouse Maternal Immune Activation
Objective: Assessment of behavioral outcomes in offspring of IL-6 knockout mice subjected to maternal immune activation compared to wild-type controls, measuring prepulse inhibition, latent inhibition, exploratory behavior, and social deficits
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Materials3
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Protocol Steps
Maternal immune activation induction
Single maternal injection of IL-6 administered to pregnant dams
Note: Injection performed on gestational day 12.5 of mouse pregnancy
View evidence from paper
“A single maternal injection of IL-6 on day 12.5 of mouse pregnancy causes prepulse inhibition (PPI) and latent inhibition (LI) deficits in the adult offspring”
Behavioral testing in adult offspring
Assessment of prepulse inhibition (PPI) and latent inhibition (LI) deficits in adult offspring
Note: Testing performed in adult offspring following maternal IL-6 injection
View evidence from paper
“A single maternal injection of IL-6 on day 12.5 of mouse pregnancy causes prepulse inhibition (PPI) and latent inhibition (LI) deficits in the adult offspring”
Anti-IL-6 antibody coadministration in poly(I:C) model
Coadministration of anti-IL-6 antibody with poly(I:C) to assess prevention of behavioral deficits
Note: Designed to prevent PPI, LI, exploratory and social deficits caused by poly(I:C)
View evidence from paper
“coadministration of an anti-IL-6 antibody in the poly(I:C) model of MIA prevents the PPI, LI, and exploratory and social deficits caused by poly(I:C)”
Maternal immune activation in IL-6 knockout mice
Induction of MIA in IL-6 knockout mice to assess behavioral outcomes compared to wild-type
Note: MIA in IL-6 knockout mice does not result in several behavioral changes seen in wild-type offspring
View evidence from paper
“MIA in IL-6 knock-out mice does not result in several of the behavioral changes seen in the offspring of wild-type mice after MIA”
Gene expression analysis
Assessment of changes in gene expression in brains of adult offspring
Note: Anti-IL-6 antibody coadministration normalizes associated changes in gene expression
View evidence from paper
“coadministration of an anti-IL-6 antibody in the poly(I:C) model of MIA prevents the PPI, LI, and exploratory and social deficits caused by poly(I:C) and normalizes the associated changes in gene expression in the brains of adult offspring”