In vivo phenotyping
Objective: Assessment of physiological and behavioral characteristics in living mice of different ages
This is a In vivo phenotyping protocol using mouse as the model organism. The procedure involves 8 procedural steps, 1 equipment items, 3 materials. Extracted from a 2011 paper published in Scientific Reports.
Model and subjects
mouse • C57BL/6J • unknown • 13 weeks (young) or 93 weeks (old) • not specified
Study window
~103 week study window
Core workflow
Acquire mice • House mice • Provide standard diet
Primary readouts
- Physiological characteristics in young and old mice
- Behavioral characteristics in young and old mice
- Plasma biochemical markers
- Molecular markers from tissue samples
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Acquire mice
Obtain C57BL/6J mice of two age groups from vendor
Note: Two cohorts: 13 weeks old (young) and 93 weeks old (old)
View evidence from paper
“C57BL/6J mice of either 13 weeks old ("young") or 93 weeks old ("old") were purchased from Janvier (St. Berthevin, France)”
House mice
Place mice in group housing conditions
Note: Mice remain group housed throughout the study period
View evidence from paper
“Mice were group housed and received standard chow”
Provide standard diet
Feed mice standard chow with specified macronutrient composition
Note: Diet catalog #2018 contains 18% protein, 50% carbohydrate, 6.0% fat
View evidence from paper
“received standard chow (#2018, containing 18% protein, 50% carbohydrate and 6.0% fat; Harlan Laboratories, Madison, WI, USA)”
Conduct in vivo phenotyping
Perform assessment of physiological and behavioral characteristics in living mice
Note: Specific phenotyping methods not detailed in this methods section
View evidence from paper
“After in vivo phenotyping, mice were sacrificed after overnight fasting”
Fast mice overnight
Withhold food from mice for overnight period prior to sacrifice
Note: Fasting occurs before sacrifice procedure
View evidence from paper
“mice were sacrificed after overnight fasting, reaching the age of 24 or 103 weeks”
Sacrifice mice
Euthanize mice at specified ages
Note: Young mice sacrificed at 24 weeks; old mice sacrificed at 103 weeks
View evidence from paper
“mice were sacrificed after overnight fasting, reaching the age of 24 or 103 weeks”
Collect heparinized plasma
Obtain blood samples in heparinized tubes
Note: Collected immediately after sacrifice
View evidence from paper
“Heparinized plasma was taken and tissues were frozen in liquid nitrogen”
Freeze tissue samples
Preserve tissue samples in liquid nitrogen for later biochemical and molecular analyses
Note: Tissues frozen immediately after collection
View evidence from paper
“tissues were frozen in liquid nitrogen for biochemical and molecular analyses”