Inescapable Footshock
Objective: To produce aversive behaviors in mice through inescapable footshock stress and measure dysphoric responses
This is a Inescapable Footshock protocol using mouse as the model organism. The procedure involves 2 procedural steps, 1 equipment items. Extracted from a 2008 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Inescapable footshock stress administration • Measurement of aversive behaviors
Primary readouts
- Aversive behaviors induced by inescapable footshock
- Blockade of aversive responses by κ-opioid receptor antagonist
- Presence or absence of aversive behaviors in dynorphin-deficient mice
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Inescapable footshock stress administration
Mice were exposed to inescapable footshock to induce stress and aversive behaviors
Note: This stress paradigm was presumed to evoke dysphoria in mice
View evidence from paper
“repeated forced swim and inescapable footshock both produced aversive behaviors that were blocked by a κ-opioid receptor antagonist”
Measurement of aversive behaviors
Aversive behaviors produced by inescapable footshock were measured and quantified
Note: Aversive behaviors were blocked by κ-opioid receptor antagonist and absent in dynorphin-deficient mice
View evidence from paper
“inescapable footshock both produced aversive behaviors that were blocked by a κ-opioid receptor (KOR) antagonist and absent in mice lacking dynorphin”