Source Paper
Hyperactivity and Intact Hippocampus-Dependent Learning in Mice Lacking the M<sub>1</sub>Muscarinic Acetylcholine Receptor
Tsuyoshi Miyakawa, Masahisa Yamada, Alokesh Duttaroy, Jürgen Wess
Journal of Neuroscience • 2001
Inescapable Footshock Test
Objective: Evaluation of stress response and freezing behavior following inescapable footshocks in mice
This is a Inescapable Footshock Test protocol using mouse as the model organism. The procedure involves 2 procedural steps, 1 equipment items. Extracted from a 2001 paper published in Journal of Neuroscience.
Model and subjects
mouse • M1 receptor-deficient mice (M1R−/− mice) and wild-type controls • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Inescapable footshock exposure • Measurement of freezing behavior
Primary readouts
- Freezing behavior following inescapable footshocks
- Stress response indicators
- Immobilization levels
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Inescapable footshock exposure
Mice were subjected to inescapable footshocks as a stressor
Note: This test was part of a battery of behavioral tests to assess stress response
View evidence from paper
“M1R−/− mice showed reduced levels of freezing after inescapable footshocks, suggesting that M1R−/− mice are hyperactive under stressful conditions”
Measurement of freezing behavior
Freezing behavior was measured and quantified following the inescapable footshock exposure
Note: Reduced freezing in M1R−/− mice was observed compared to controls
View evidence from paper
“M1R−/− mice showed reduced levels of freezing after inescapable footshocks”