Source Paper
Short-Chain Fatty Acids Stimulate Glucagon-Like Peptide-1 Secretion via the G-Protein–Coupled Receptor FFAR2
Gwen Tolhurst, Helen Heffron, Yu Shan Lam, Helen E. Parker, Abdella M. Habib et al.
Diabetes • 2011
Insulin Tolerance Test
Objective: To assess insulin sensitivity in fasted ffar2 and ffar3 knockout mice and wild-type controls by measuring blood glucose response to insulin injection over time
This is a Insulin Tolerance Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 2 materials. Extracted from a 2011 paper published in Diabetes.
Model and subjects
mouse • 129/SvEv • unknown • 3-4 months • approximately 20-23g
Study window
~8 hours hands-on
Core workflow
Fasting period • Insulin injection • Blood glucose measurement
Primary readouts
- Blood glucose levels measured at multiple time points following insulin injection
- Insulin sensitivity calculated from glucose clearance rate
Key equipment and reagents
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Protocol Steps
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Fasting period
Mice were fasted for 4 hours prior to insulin injection
Note: Fasting period to establish baseline metabolic state
View evidence from paper
“Three- to four-month-old ffar2 −/−, ffar3 −/−, and wild-type littermate control 129/SvEv mice were fasted for 4 h”
Insulin injection
Mice were dosed with insulin via intraperitoneal injection at 0.75 units/kg
Note: Insulin supplied by Named Veterinary Surgeon; intraperitoneal route
View evidence from paper
“dosed with 0.75 units/kg insulin i.p. (Actrapid insulin, supplied by our Named Veterinary Surgeon)”
Blood glucose measurement
Blood glucose was measured from awake mice via tail bleed using handheld glucometer at specified time points
Note: Measurements taken from awake mice without anesthesia; specific time points not explicitly stated in provided text
View evidence from paper
“Blood glucose was measured using a handheld glucometer (OneTouch Ultra) via tail bleed from awake mice”