Source Paper
Genomic Analysis of Reactive Astrogliosis
Jennifer L. Zamanian, Lijun Xu, Lynette C. Foo, Navid Nouri, Lu Zhou et al.
Journal of Neuroscience • 2012
Ischemic Stroke Model
Objective: To induce reactive astrogliosis in mice using an ischemic stroke injury model for subsequent gene expression analysis and astrocyte phenotype characterization
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Software1
Affymetrix • Not specified in provided text
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Protocol Steps
Ischemic Stroke Induction
Induce ischemic stroke injury in mice to trigger reactive astrogliosis
Note: This is one of two injury models used in the study; the other being neuroinflammation
View evidence from paper
“we used Affymetrix GeneChip arrays to profile gene expression in populations of reactive astrocytes isolated at various time points after induction using two mouse injury models, ischemic stroke and neuroinflammation”
Astrocyte Isolation
Isolate reactive astrocyte populations at various time points following ischemic stroke induction
Note: Astrocytes were collected at multiple time points to capture the temporal dynamics of reactive gliosis
View evidence from paper
“populations of reactive astrocytes isolated at various time points after induction using two mouse injury models, ischemic stroke and neuroinflammation”
Gene Expression Profiling
Profile gene expression in isolated reactive astrocytes using Affymetrix GeneChip arrays
Note: Analysis identifies reactive astrocyte markers and characterizes phenotype-specific gene expression
View evidence from paper
“we used Affymetrix GeneChip arrays to profile gene expression in populations of reactive astrocytes”