Source Paper
Genomic Analysis of Reactive Astrogliosis
Jennifer L. Zamanian, Lijun Xu, Lynette C. Foo, Navid Nouri, Lu Zhou et al.
Journal of Neuroscience • 2012
Ischemic Stroke Model
Objective: To induce reactive astrogliosis in mice using an ischemic stroke injury model for subsequent gene expression analysis and astrocyte phenotype characterization
This is a Ischemic Stroke Model protocol using mouse as the model organism. The procedure involves 3 procedural steps. Extracted from a 2012 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Ischemic Stroke Induction • Astrocyte Isolation • Gene Expression Profiling
Primary readouts
- Gene expression profiles in reactive astrocytes
- Identification of Lcn2 and Serpina3n as markers of reactive astrocytes
- Characterization of injury-type-specific gene expression patterns
- Molecular phenotype of reactive astrocytes in ischemia versus neuroinflammation
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Ischemic Stroke Induction
Induce ischemic stroke injury in mice to trigger reactive astrogliosis
Note: This is one of two injury models used in the study; the other being neuroinflammation
View evidence from paper
“we used Affymetrix GeneChip arrays to profile gene expression in populations of reactive astrocytes isolated at various time points after induction using two mouse injury models, ischemic stroke and neuroinflammation”
Astrocyte Isolation
Isolate reactive astrocyte populations at various time points following ischemic stroke induction
Note: Astrocytes were collected at multiple time points to capture the temporal dynamics of reactive gliosis
View evidence from paper
“populations of reactive astrocytes isolated at various time points after induction using two mouse injury models, ischemic stroke and neuroinflammation”
Gene Expression Profiling
Profile gene expression in isolated reactive astrocytes using Affymetrix GeneChip arrays
Note: Analysis identifies reactive astrocyte markers and characterizes phenotype-specific gene expression
View evidence from paper
“we used Affymetrix GeneChip arrays to profile gene expression in populations of reactive astrocytes”