JAK Inhibitor Treatment in Aged Mice
Objective: To assess the effects of JAK inhibitor treatment on inflammation and physical function in aged mice over a 10-week treatment period
This is a JAK Inhibitor Treatment in Aged Mice protocol using mouse as the model organism. The procedure involves 4 procedural steps, 2 materials. Extracted from a 2015 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
mouse • Not specified in provided text • unknown • aged • Not specified in provided text
Study window
~10 week study window
Core workflow
JAK Inhibitor Administration • Assessment of Adipose Tissue Inflammation • Assessment of Systemic Inflammation
Primary readouts
- Adipose tissue inflammation levels
- Systemic inflammation markers
- Physical function and frailty assessment
- Senescence-associated secretory phenotype (SASP) markers
Key equipment and reagents
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Protocol Steps
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JAK Inhibitor Administration
Administer JAK inhibitor to aged mice
Note: Treatment period designed to assess effects on inflammation and physical function
View evidence from paper
“The administration of JAK inhibitor to aged mice for 10 wk alleviated both adipose tissue and systemic inflammation and enhanced physical function”
Assessment of Adipose Tissue Inflammation
Measure inflammation markers in adipose tissue following JAK inhibitor treatment
Note: Adipose tissue inflammation is a key outcome measure
View evidence from paper
“The administration of JAK inhibitor to aged mice for 10 wk alleviated both adipose tissue and systemic inflammation”
Assessment of Systemic Inflammation
Measure systemic inflammation markers following JAK inhibitor treatment
Note: Systemic inflammation is a key outcome measure
View evidence from paper
“The administration of JAK inhibitor to aged mice for 10 wk alleviated both adipose tissue and systemic inflammation”
Assessment of Physical Function
Evaluate physical function and frailty in aged mice following JAK inhibitor treatment
Note: Physical function enhancement is a primary outcome measure
View evidence from paper
“The administration of JAK inhibitor to aged mice for 10 wk alleviated both adipose tissue and systemic inflammation and enhanced physical function”