Source Paper
Disruption of the Metallothionein-III Gene in Mice: Analysis of Brain Zinc, Behavior, and Neuron Vulnerability to Metals, Aging, and Seizures
Jay C. Erickson, Gunther Hollopeter, Steven A. Thomas, Glenda J. Froelick, Richard D. Palmiter
Journal of Neuroscience • 1997
Kainic Acid-Induced Seizure Susceptibility
Objective: Evaluate seizure susceptibility and neuron injury in the CA3 field of hippocampus following kainic acid injection in MT-III-deficient mice compared to wild-type controls
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Equipment1
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Materials1
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Protocol Steps
Kainic acid injection and seizure induction
Administer kainic acid to induce seizures in MT-III-deficient mice and control mice
Note: MT-III-deficient mice were more susceptible to seizures induced by kainic acid
View evidence from paper
“MT-III-deficient mice were more susceptible to seizures induced by kainic acid and subsequently exhibited greater neuron injury in the CA3 field of hippocampus”
Assessment of neuron injury
Evaluate neuron injury in the CA3 field of hippocampus following kainic acid-induced seizures
Note: MT-III-deficient mice exhibited greater neuron injury in CA3 field compared to controls
View evidence from paper
“MT-III-deficient mice were more susceptible to seizures induced by kainic acid and subsequently exhibited greater neuron injury in the CA3 field of hippocampus”
Morris water maze testing
Test spatial learning in Morris water maze to assess cognitive function
Note: Mutant mice exhibited normal spatial learning
View evidence from paper
“Mutant mice exhibited normal spatial learning in the Morris water maze”