Source Paper
Disruption of the Metallothionein-III Gene in Mice: Analysis of Brain Zinc, Behavior, and Neuron Vulnerability to Metals, Aging, and Seizures
Jay C. Erickson, Gunther Hollopeter, Steven A. Thomas, Glenda J. Froelick, Richard D. Palmiter
Journal of Neuroscience • 1997
Kainic Acid-Induced Seizure Susceptibility
Objective: Evaluate seizure susceptibility and neuron injury in the CA3 field of hippocampus following kainic acid injection in MT-III-deficient mice compared to wild-type controls
This is a Kainic Acid-Induced Seizure Susceptibility protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 1 materials. Extracted from a 1997 paper published in Journal of Neuroscience.
Model and subjects
mouse • MT-III-deficient mice and transgenic mice with elevated MT-III levels • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Kainic acid injection and seizure induction • Assessment of neuron injury • Morris water maze testing
Primary readouts
- Seizure susceptibility following kainic acid injection
- Neuron injury in CA3 field of hippocampus
- Spatial learning performance in Morris water maze
- Brain zinc concentrations in hippocampus and other brain regions
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Kainic acid injection and seizure induction
Administer kainic acid to induce seizures in MT-III-deficient mice and control mice
Note: MT-III-deficient mice were more susceptible to seizures induced by kainic acid
View evidence from paper
“MT-III-deficient mice were more susceptible to seizures induced by kainic acid and subsequently exhibited greater neuron injury in the CA3 field of hippocampus”
Assessment of neuron injury
Evaluate neuron injury in the CA3 field of hippocampus following kainic acid-induced seizures
Note: MT-III-deficient mice exhibited greater neuron injury in CA3 field compared to controls
View evidence from paper
“MT-III-deficient mice were more susceptible to seizures induced by kainic acid and subsequently exhibited greater neuron injury in the CA3 field of hippocampus”
Morris water maze testing
Test spatial learning in Morris water maze to assess cognitive function
Note: Mutant mice exhibited normal spatial learning
View evidence from paper
“Mutant mice exhibited normal spatial learning in the Morris water maze”