Latent Inhibition Test
Objective: Evaluation of behavioral and cognitive flexibility through measurement of latent inhibition effects in offspring exposed to prenatal immune challenge
This is a Latent Inhibition Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 2 materials. Extracted from a 2010 paper published in Neuropsychopharmacology.
Model and subjects
mouse • Not specified in provided text • both • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Prenatal Immune Challenge Administration • Latent Inhibition Testing • Acute Apomorphine Treatment and Behavioral Assessment
Primary readouts
- Latent inhibition effect magnitude
- Behavioral/cognitive flexibility
- Locomotor responses to apomorphine
- Stereotyped behavioral responses to apomorphine
Key equipment and reagents
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Protocol Steps
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Prenatal Immune Challenge Administration
Pregnant dams receive intravenous injection of Poly-I:C on gestation day 17
Note: Administered to pregnant mothers; offspring subsequently tested for behavioral outcomes
View evidence from paper
“prenatal exposure to the viral mimic polyriboinosinic-polyribocytidilic acid (Poly-I:C; 5 mg/kg, i.v.) on gestation day (GD) 17”
Latent Inhibition Testing
Offspring undergo latent inhibition testing to assess behavioral and cognitive flexibility
Note: Testing conducted on both male and female offspring; abnormally enhanced latent inhibition effect observed in males but not females
View evidence from paper
“male but not female offspring born to immune challenged mothers displayed behavioral/cognitive inflexibility as indexed by the presence of an abnormally enhanced latent inhibition (LI) effect”
Acute Apomorphine Treatment and Behavioral Assessment
Offspring receive acute apomorphine treatment and are assessed for locomotor and stereotyped behavioral responses
Note: Alterations observed in both male and female offspring
View evidence from paper
“alterations in the locomotor and stereotyped behavioral responses to acute apomorphine (APO) treatment in both male and female offspring”