Latent Inhibition Test
Objective: Evaluation of behavioral and cognitive flexibility through measurement of latent inhibition effects in offspring exposed to prenatal immune challenge
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified in provided text • Not specified • Not specified • Not mentioned
Materials2
Software1
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Protocol Steps
Prenatal Immune Challenge Administration
Pregnant dams receive intravenous injection of Poly-I:C on gestation day 17
Note: Administered to pregnant mothers; offspring subsequently tested for behavioral outcomes
View evidence from paper
“prenatal exposure to the viral mimic polyriboinosinic-polyribocytidilic acid (Poly-I:C; 5 mg/kg, i.v.) on gestation day (GD) 17”
Latent Inhibition Testing
Offspring undergo latent inhibition testing to assess behavioral and cognitive flexibility
Note: Testing conducted on both male and female offspring; abnormally enhanced latent inhibition effect observed in males but not females
View evidence from paper
“male but not female offspring born to immune challenged mothers displayed behavioral/cognitive inflexibility as indexed by the presence of an abnormally enhanced latent inhibition (LI) effect”
Acute Apomorphine Treatment and Behavioral Assessment
Offspring receive acute apomorphine treatment and are assessed for locomotor and stereotyped behavioral responses
Note: Alterations observed in both male and female offspring
View evidence from paper
“alterations in the locomotor and stereotyped behavioral responses to acute apomorphine (APO) treatment in both male and female offspring”