Lifespan Measurement
Objective: Measurement of overall lifespan in mice with altered IGF-1 levels at different developmental stages (perinatal, post-pubertal, and late-adult onset)
Protocol Steps
Animal Model Generation
Utilized igf f/f C57Bl/6 mice for genetic manipulation of IGF-1 levels at multiple developmental stages
Note: Both genetic and viral approaches were used to induce IGF-1 deficiency
View evidence from paper
“investigated the effects of perinatal, post-pubertal, and late-adult onset IGF-1 deficiency using genetic and viral approaches in both male and female igf f/f C57Bl/6 mice”
Lifespan Monitoring
Monitored mice throughout their lifespan to measure overall survival and longevity outcomes
Note: Measurements included overall lifespan, healthspan, and pathology assessment
View evidence from paper
“examined how alterations in IGF-1 levels at multiple stages of development and aging influence overall lifespan, healthspan, and pathology”
Pathology Assessment
Evaluated pathological changes and tissue health across different tissues and sexes throughout the lifespan
Note: Changes were assessed as specific for each sex and tissue
View evidence from paper
“these changes are specific for each sex and tissue”
Late-Life Analysis
Specifically examined effects of late-life IGF-1 deficiency on cancer risk and lifespan extension
Note: Late-life IGF-1 deficiency was identified as a time point relevant for human studies
View evidence from paper
“late-life IGF-1 deficiency (a time point relevant for human studies) reduces cancer risk but does not increase lifespan”