Lifespan Studies

“Experiments were performed on male nfkb1 −/− mice on a pure C57Bl/6 background and C57Bl/6 wt controls. Pure background mice were a gift from Jorge Caamano, (Birmingham University, UK)”

“Mice were housed in cages (56 cm × 38 cm × 18 cm, North Kent Plastics, Kent, UK) of groups of 4–6 that did not change from weaning. Mice were provided with saw dust and paper bedding and had AL access to water”

“Mice were housed at 20±2 °C under a 12 h light/12 h dark photoperiod with lights on at 07:00 hours”

“Lifespan studies were also performed in male C57Bl/6 under ad libitum feeding (AL) and under dietary restriction (DR, 60% of AL intake)”

“Group sizes for lifespan experiments were (censored events in brackets): nfkb1 −/− 27 (18), C57Bl/6 AL 310 (172), C57Bl/6 DR 241 (157), IRCFa 2391 (1,061) and F3TERC −/− 13 (0)”

“Organs were either fixed in 4% paraformaldehyde or snap-frozen in liquid nitrogen and stored at −80 °C”

“Fixed tissues were processed and embedded in paraffin. All sections were cut at a thickness of 3 μm”

“Telomerase activity was measured by TeloTAGGG Telomerase PCR ELISA kit (Roche)”

“70% partial hepatectomy (PHX) was performed in 12–week-old mice according to the method of Higgins and Anderson”