Locomotor Activity Assessment
Objective: Measurement of horizontal and vertical locomotor activity in mice during assessment of behavioral deficits induced by anti-NMDAR antibodies
This is a Locomotor Activity Assessment protocol using mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 3 materials. Extracted from a 2014 paper published in Brain.
Model and subjects
mouse • C57BL6/J • unknown • Not specified • Not specified
Study window
~2 week study window
Core workflow
Surgical implantation of ventricular catheters and osmotic pumps • Continuous cerebrospinal fluid infusion • Locomotor activity assessment
Primary readouts
- Horizontal locomotor activity
- Vertical locomotor activity
- Memory deficits (novel object recognition, V-maze performance)
- Anhedonic behaviors (sucrose preference)
Key equipment and reagents
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Protocol Steps
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Surgical implantation of ventricular catheters and osmotic pumps
C57BL6/J mice underwent surgical placement of ventricular catheters connected to osmotic pumps for continuous cerebrospinal fluid delivery
Note: Catheters and pumps remained in place for 14-day infusion period
View evidence from paper
“C57BL6/J mice underwent placement of ventricular catheters connected to osmotic pumps that delivered a continuous infusion”
Continuous cerebrospinal fluid infusion
Osmotic pumps delivered continuous infusion of either patients' cerebrospinal fluid or control cerebrospinal fluid at controlled flow rate
Note: Flow rate maintained at 0.25 µl/h throughout infusion period
View evidence from paper
“osmotic pumps that delivered a continuous infusion of patients' or control cerebrospinal fluid (flow rate 0.25 µl/h, 14 days)”
Locomotor activity assessment
Measurement of horizontal and vertical locomotor activity using standardized testing apparatus during and after infusion period
Note: Testing conducted as part of comprehensive behavioral assessment battery
View evidence from paper
“During and after the infusion period standardized tests were applied, including tasks to assess memory (novel object recognition in open field and V-maze paradigms), anhedonic behaviours (sucrose preference test), depressive-like behaviours (tail suspension, forced swimming tests), anxiety (black and white, elevated plus maze tests), aggressiveness (resident-intruder test), and locomotor activity (horizontal and vertical)”
Animal sacrifice and tissue collection
Animals were sacrificed at specified timepoints for brain tissue analysis
Note: Sacrifice occurred at Days 5, 13, 18, 26, and 46 post-infusion initiation
View evidence from paper
“Animals sacrificed at Days 5, 13, 18, 26 and 46 were examined for brain-bound antibodies and the antibody effects on total and synaptic NMDAR clusters”