Locomotor Activity in Novel Environment
Objective: Measurement of locomotor activity in a novel environment as an index of anxiety-like behavior in CRF transgenic mice compared to normal littermate controls
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Equipment2
Materials1
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Protocol Steps
Subject preparation
CRF transgenic mice and normal littermate controls were prepared for testing
Note: Both transgenic and control animals were tested
View evidence from paper
“CRF transgenic mice and normal littermate controls were tested by measuring locomotor activity in a novel environment”
Locomotor activity measurement in novel environment
Animals were placed in a novel environment and locomotor activity was measured as an index of anxiety-like behavior
Note: This measurement served as one of two anxiety indices
View evidence from paper
“measuring locomotor activity in a novel environment and through the use of an elevated plus-maze as indices of anxiety”
Elevated plus-maze testing
Animals were tested on an elevated plus-maze as a second index of anxiety-like behavior
Note: This was used in conjunction with locomotor activity measurement
View evidence from paper
“through the use of an elevated plus-maze as indices of anxiety”
CRF antagonist injection
CRF antagonist alpha-helical CRF 9-41 was injected into the lateral cerebral ventricles of CRF transgenic animals
Note: This injection was performed to test whether central CRF overproduction mediated the anxiogenic behavior
View evidence from paper
“Injection of the CRF antagonist alpha-helical CRF 9-41 into the lateral cerebral ventricles reversed the anxiogenic state observed in the CRF transgenics”