Locomotor Activity Test
Objective: Assessment of baseline locomotor activity and general motor function in mice with hippocampus-specific BDNF deletion
This is a Locomotor Activity Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 2 materials. Extracted from a 2007 paper published in Molecular Psychiatry.
Model and subjects
mouse • Not explicitly stated • Not explicitly stated • adult • Not explicitly stated • Not explicitly stated
Study window
Estimated timing pending
Core workflow
Lentivirus injection • Locomotor activity assessment • Baseline anxiety measurement
Primary readouts
- Baseline locomotor activity
- General motor function
- Baseline anxiety (startle response)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Lentivirus injection
Bilateral injection of lentivirus expressing Cre recombinase into the dorsal hippocampus of adult mice floxed at the BDNF locus
Note: Site-specific deletion of BDNF gene in adult animals
View evidence from paper
“we injected a lentivirus expressing Cre recombinase bilaterally into the dorsal hippocampus in adult mice floxed at the BDNF locus”
Locomotor activity assessment
Measurement of baseline locomotion in animals with BDNF deletion compared to control animals
Note: No significant effects of BDNF deletion on locomotion were found
View evidence from paper
“there were no significant effects of BDNF deletion on locomotion or baseline anxiety measured with startle”
Baseline anxiety measurement
Assessment of baseline anxiety using startle response
Note: No significant effects of BDNF deletion on baseline anxiety
View evidence from paper
“baseline anxiety measured with startle”