Locomotor Activity Test
Objective: Assessment of baseline locomotor activity and general motor function in mice with hippocampus-specific BDNF deletion
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Equipment1
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Materials2
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Protocol Steps
Lentivirus injection
Bilateral injection of lentivirus expressing Cre recombinase into the dorsal hippocampus of adult mice floxed at the BDNF locus
Note: Site-specific deletion of BDNF gene in adult animals
View evidence from paper
“we injected a lentivirus expressing Cre recombinase bilaterally into the dorsal hippocampus in adult mice floxed at the BDNF locus”
Locomotor activity assessment
Measurement of baseline locomotion in animals with BDNF deletion compared to control animals
Note: No significant effects of BDNF deletion on locomotion were found
View evidence from paper
“there were no significant effects of BDNF deletion on locomotion or baseline anxiety measured with startle”
Baseline anxiety measurement
Assessment of baseline anxiety using startle response
Note: No significant effects of BDNF deletion on baseline anxiety
View evidence from paper
“baseline anxiety measured with startle”