Source Paper
Effects of Cardamom on Neuroinflammation, Learning and Memory in Mice Fed a Cafeteria Diet
AL‐Dalaeen A, Batarseh N, Atawneh S
Endocrinol Diabetes Metab • 2026
Marble Burying Test
behavioralMouseC57BL/6
Objective: To assess anxiety and repetitive behavior in mice
Materials & Equipment Checklist
4 items2 from ConductScience
Gather these items before starting the experiment. Check off items as you prepare.
2 items available from ConductScience
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Protocol Steps
1
Setup marble arrangement
Place fifty glass marbles evenly spaced on 5 cm deep bedding in a standard cage
View evidence from paper
“Fifty glass marbles were evenly spaced on 5 cm deep bedding in a standard cage”
2
Place mouse in cage
Place the mouse in the cage containing the arranged marbles
View evidence from paper
“Mice were placed in the cage for 30 min”
3
Allow exploration period
Allow the mouse to explore and interact with marbles for 30 minutes
View evidence from paper
“Mice were placed in the cage for 30 min”
4
Count buried marbles
Count the number of marbles that were buried to at least two-thirds depth
View evidence from paper
“the number of marbles buried (to at least two‐thirds depth) was counted”