Source Paper
Effects of Cardamom on Neuroinflammation, Learning and Memory in Mice Fed a Cafeteria Diet
AL‐Dalaeen A, Batarseh N, Atawneh S
Endocrinol Diabetes Metab • 2026
Marble Burying Test
Objective: To assess anxiety and repetitive behavior in mice
This is a Marble Burying Test protocol using Mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 3 materials. Extracted from a 2026 paper published in Endocrinol Diabetes Metab.
Model and subjects
Mouse • C57BL/6 • Male • 6-week-old • 36
Study window
~30 minutes hands-on
Core workflow
Setup marble arrangement • Place mouse in cage • Allow exploration period
Primary readouts
- Number of marbles buried to at least two-thirds depth
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Setup marble arrangement
Place fifty glass marbles evenly spaced on 5 cm deep bedding in a standard cage
View evidence from paper
“Fifty glass marbles were evenly spaced on 5 cm deep bedding in a standard cage”
Place mouse in cage
Place the mouse in the cage containing the arranged marbles
View evidence from paper
“Mice were placed in the cage for 30 min”
Allow exploration period
Allow the mouse to explore and interact with marbles for 30 minutes
View evidence from paper
“Mice were placed in the cage for 30 min”
Count buried marbles
Count the number of marbles that were buried to at least two-thirds depth
View evidence from paper
“the number of marbles buried (to at least two‐thirds depth) was counted”