Marble Burying Test
Objective: Assessment of repetitive and anxiety-related behavior through marble burying in mice
This is a Marble Burying Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 2 materials. Extracted from a 2016 paper published in Molecular Psychiatry.
Model and subjects
mouse • C57BL/6J background • male • 60-90 days • 101
Study window
~30 minutes hands-on
Core workflow
Animal acclimation to testing room • Marble burying test execution • Baseline behavioral testing completion
Primary readouts
- Number of marbles buried
- Repetitive behavior assessment
- Anxiety-related behavior indicators
Key equipment and reagents
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Protocol Steps
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Animal acclimation to testing room
Animals were given habituation time to the behavioral testing room prior to testing
Note: Habituation performed before behavioral phenotyping
View evidence from paper
“Animals were given 30 min of habituation to the behavioral testing room”
Marble burying test execution
Marble burying test performed as part of behavioral phenotyping sequence, conducted after rotarod and elevated plus maze tests but before open field test
Note: Tests performed from least to most invasive; testing window between 0900h and 1600h
View evidence from paper
“Behavioral phenotyping was performed between 0900 h and 1600 h. Tests were performed from the least to the most invasive to minimize the influence of prior test history (in order: rotarod, elevated plus maze, marble burying test, open field test, sucrose preference test, novelty suppressed feeding and forced swim test)”
Baseline behavioral testing completion
Complete baseline behavioral testing battery including marble burying test prior to chronic restraint stress
Note: Baseline testing performed before 21-day restraint stress protocol
View evidence from paper
“After baseline behavioral testing, animals were submitted to restraint stress for 21 days”