Marble Burying Test
behavioralmouseC57BL/6J background
Objective: Assessment of repetitive and anxiety-related behavior through marble burying in mice
Materials & Equipment Checklist
3 items
Protocol Steps
1
Animal acclimation to testing room
Animals were given habituation time to the behavioral testing room prior to testing
30 minutes
Note: Habituation performed before behavioral phenotyping
View evidence from paper
“Animals were given 30 min of habituation to the behavioral testing room”
2
Marble burying test execution
Marble burying test performed as part of behavioral phenotyping sequence, conducted after rotarod and elevated plus maze tests but before open field test
Note: Tests performed from least to most invasive; testing window between 0900h and 1600h
View evidence from paper
“Behavioral phenotyping was performed between 0900 h and 1600 h. Tests were performed from the least to the most invasive to minimize the influence of prior test history (in order: rotarod, elevated plus maze, marble burying test, open field test, sucrose preference test, novelty suppressed feeding and forced swim test)”
3
Baseline behavioral testing completion
Complete baseline behavioral testing battery including marble burying test prior to chronic restraint stress
Note: Baseline testing performed before 21-day restraint stress protocol
View evidence from paper
“After baseline behavioral testing, animals were submitted to restraint stress for 21 days”