Mechanical Allodynia Testing
Objective: Measurement of pain sensitivity using mechanical stimulation in astrocyte-specific trkB.T1 knockout mice after spinal cord injury to assess mechanical allodynia and hyperpathic responses
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Protocol Steps
Mechanical Allodynia Testing
Conduct mechanical allodynia testing on astrocyte-specific trkB.T1 knockout mice after spinal cord injury using the CatWalk system to measure pain sensitivity and hyperpathic responses
Note: Testing was performed on both WT and KO mice to compare hyperpathic responses; KO mice showed reduced hyperpathic responses compared to WT controls
View evidence from paper
“using mechanical allodynia and pain-related measurements on the CatWalk, these animals also showed reduced hyperpathic responses, along with improved motor coordination”