Memory Loss Assessment
Objective: Assess memory loss in a conditional mouse model of tauopathy (rTg4510) and correlate tau protein species accumulation with behavioral deficits at various ages
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Protocol Steps
Biochemical analysis of brain tissue
Perform biochemical analyses on brain tissues from the rTg4510 mouse model to identify tau protein species
Note: Analysis aimed at identifying neurotoxic tau protein species
View evidence from paper
“performed biochemical analyses on brain tissues from the rTg4510 mouse model and then correlated the levels of these tau proteins with memory loss”
Identification of tau multimers
Identify and characterize two forms of tau multimers with molecular weights of 140 and 170 kDa, suggesting oligomeric aggregates
Note: These multimers accumulate early in the pathogenic cascade
View evidence from paper
“describe the identification and characterization of two forms of tau multimers (140 and 170 kDa), whose molecular weight suggests an oligomeric aggregate, that accumulate early in the pathogenic cascade”
Behavioral assessment of memory loss
Assess memory loss in rTg4510 mice at various ages
Note: Memory loss assessment correlated with tau protein accumulation
View evidence from paper
“correlated the levels of these tau proteins with memory loss”
Comparative analysis across models
Detect and compare tau multimers in JNPL3 mouse model and human tissue samples from Alzheimer's disease and FTDP-17 patients
Note: Validation of findings across multiple tauopathy models
View evidence from paper
“Similar tau multimers were detected in a second mouse model of tauopathy (JNPL3) and in tissue from patients with Alzheimer's disease and FTDP-17”
Correlation analysis
Correlate levels of tau multimers with memory loss at various ages in the rTg4510 mouse model
Note: Consistent correlation between tau multimer levels and memory deficits
View evidence from paper
“levels of the tau multimers correlated consistently with memory loss at various ages in the rTg4510 mouse model”