Middle Cerebral Artery Occlusion
Objective: To investigate the impact of BDNF Val66Met polymorphism on motor recovery following transient middle cerebral artery occlusion-induced ischemic stroke in mice
Protocol Steps
Surgical procedure - Transient middle cerebral artery occlusion
Male BDNF +/+ and BDNF M/M littermates received either sham surgery or transient middle cerebral artery occlusion
Note: The text does not provide detailed surgical methodology, anesthesia protocols, or specific occlusion duration
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“Male BDNF +/+ and BDNF M/M littermates received sham or transient middle cerebral artery occlusion”
Motor function assessment
Motor function was assessed regularly for 6 months after stroke
Note: Specific behavioral tests and assessment methods are not detailed in the provided text
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“We assessed motor function regularly for 6 months after stroke and then performed anatomical analyses”
Anatomical analyses
Anatomical analyses were performed after the 6-month motor function assessment period
Note: Specific anatomical methods are not detailed in the provided text
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“We assessed motor function regularly for 6 months after stroke and then performed anatomical analyses”
Transient inactivation of contralateral striatum
Transient inactivation of the contralateral striatum was performed during recovery to test its role in enhanced motor function
Note: Specific methodology for inactivation is not detailed in the provided text
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“Transient inactivation of the contralateral striatum during recovery transiently abolished the enhanced function”