Morris Water Maze - Classic Version
Objective: Measure spatial memory for a fixed platform location in the Morris water maze to assess cognitive performance in enriched versus standard-housed mice, particularly in transgenic Alzheimer's disease models
This is a Morris Water Maze - Classic Version protocol using mouse as the model organism. The procedure involves 3 procedural steps, 2 equipment items. Extracted from a 2005 paper published in Journal of Neuroscience.
Model and subjects
mouse • Transgenic mice overexpressing amyloid precursor protein and/or presenilin-1 and nontransgenic controls • unknown • 2 months at start of housing, tested after 6 months of differential housing
Study window
Estimated timing pending
Core workflow
Housing Assignment • Classic Morris Water Maze Testing • Repeated-Reversals Morris Water Maze Testing
Primary readouts
- Performance in classic Morris water maze (memory for fixed platform location)
- Performance in repeated-reversals Morris water maze (learning of new platform positions)
- Performance in radial water maze (spatial memory)
- Comparison between enriched and standard-housed mice
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Housing Assignment
Female transgenic mice overexpressing amyloid precursor protein and/or presenilin-1 and nontransgenic controls were placed into enriched or standard cages at 2 months of age
Note: Mice were divided into enriched and standard housing conditions
View evidence from paper
“Female transgenic mice overexpressing amyloid precursor protein and/or presenilin-1 and nontransgenic controls were placed into enriched or standard cages at 2 months of age and tested for cognitive behavior after 6 months of differential housing.”
Classic Morris Water Maze Testing
Mice were tested in the classic Morris water maze to measure memory for a fixed platform location
Note: Performance measured for platform location memory; amyloid-β overproducing mice showed weaker memory compared to nontransgenic cagemates
View evidence from paper
“Mice overproducing amyloid-β (Aβ), particularly those with amyloid deposits, showed weaker memory for the platform location in the classic Morris water maze”
Repeated-Reversals Morris Water Maze Testing
Mice were tested in repeated-reversal versions of the Morris water maze to measure learning of new platform positions
Note: Amyloid-β overproducing mice learned new platform positions less quickly than nontransgenic cagemates
View evidence from paper
“learned new platform positions in the repeated-reversals task less quickly than their nontransgenic cagemates”