Source Paper
The potential of human milk oligosaccharides in ameliorating traumatic brain injury-induced cognitive impairment in mice
He D, He R, Duan W, Li G, Kang Q
Future Sci OA • 2026
Morris Water Maze
Objective: Assessment of spatial learning and memory performance in mice following traumatic brain injury
Gather these items before starting the experiment. Check off items as you prepare.
Materials1
not specified • not specified
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Protocol Steps
Pool setup
Set up black circular pool with four distinct markers on walls of quadrants. Place transparent platform 15 cm from southwest wall and 25 cm from pool bottom. Maintain water temperature at 25°C with water level 3 cm above platform.
View evidence from paper
“there are four distinct markers on the walls of the four quadrants of the pool”
Training phase
Place mice into pool from any quadrant, conduct 4 training sessions per day. When mouse reaches platform and stays for 5 seconds, or manually place on platform for 5 seconds if 90-second limit expires, then remove and dry mouse.
View evidence from paper
“In the training phase, mice are placed into the pool from any one of the four quadrants every day, with 4 training sessions per day”
Test phase
Remove platform and record time taken for mice to reach original platform position (escape latency) and number of platform crossings after entering water.
View evidence from paper
“In the test phase, the platform is removed, and the time taken for the mice to reach the original position of the platform”