Source Paper
The potential of human milk oligosaccharides in ameliorating traumatic brain injury-induced cognitive impairment in mice
He D, He R, Duan W, Li G, Kang Q
Future Sci OA • 2026
Morris Water Maze
Objective: Assessment of spatial learning and memory performance in mice following traumatic brain injury
This is a Morris Water Maze protocol using mouse as the model organism. The procedure involves 3 procedural steps, 2 equipment items, 1 materials. Extracted from a 2026 paper published in Future Sci OA.
Model and subjects
mouse • C57BL/6 • not specified • 6–8 weeks old • 48
Study window
Estimated timing pending
Core workflow
Pool setup • Training phase • Test phase
Primary readouts
- Escape latency (time to reach original platform position)
- Crossing times (number of times crossing platform location)
- Swimming trajectory
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Pool setup
Set up black circular pool with four distinct markers on walls of quadrants. Place transparent platform 15 cm from southwest wall and 25 cm from pool bottom. Maintain water temperature at 25°C with water level 3 cm above platform.
View evidence from paper
“there are four distinct markers on the walls of the four quadrants of the pool”
Training phase
Place mice into pool from any quadrant, conduct 4 training sessions per day. When mouse reaches platform and stays for 5 seconds, or manually place on platform for 5 seconds if 90-second limit expires, then remove and dry mouse.
View evidence from paper
“In the training phase, mice are placed into the pool from any one of the four quadrants every day, with 4 training sessions per day”
Test phase
Remove platform and record time taken for mice to reach original platform position (escape latency) and number of platform crossings after entering water.
View evidence from paper
“In the test phase, the platform is removed, and the time taken for the mice to reach the original position of the platform”