Source Paper
Requirement for Hippocampal CA3 NMDA Receptors in Associative Memory Recall
Kazu Nakazawa, Michael C. Quirk, Raymond A. Chitwood, Masahiko Watanabe, Mark F. Yeckel et al.
Science • 2002
Morris Water Maze
Objective: Assess spatial reference memory acquisition and retrieval in mutant mice under full and partial cue conditions to test CA3 NMDA receptor involvement in associative memory recall
This is a Morris Water Maze protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 2002 paper published in Science.
Model and subjects
mouse • genetically engineered with NMDA receptor gene ablated in CA3 pyramidal cells • not specified • adult • not specified • not specified
Study window
Estimated timing pending
Core workflow
Spatial Reference Memory Acquisition • Full Cue Retrieval Testing • Partial Cue Retrieval Testing
Primary readouts
- Spatial reference memory acquisition performance
- Memory retrieval performance under full cue conditions
- Memory retrieval performance under partial cue conditions
- Place-related activity in CA1 pyramidal cells in full-cue environment
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Spatial Reference Memory Acquisition
Mutant mice were trained to acquire spatial reference memory in the Morris water maze under standard conditions
Note: Normal acquisition observed in mutant mice
View evidence from paper
“mutant mice normally acquired and retrieved spatial reference memory in the Morris water maze”
Full Cue Retrieval Testing
Mice were tested for memory retrieval with all original environmental cues present
Note: Normal retrieval performance observed in mutant mice with full cues
View evidence from paper
“mutant mice normally acquired and retrieved spatial reference memory in the Morris water maze”
Partial Cue Retrieval Testing
Mice were tested for memory retrieval when presented with only a fraction of the original environmental cues
Note: Mutant mice showed impaired retrieval performance under partial cue conditions
View evidence from paper
“they were impaired in retrieving this memory when presented with a fraction of the original cues”