Source Paper
Sex-dependent effects of ultra-low-dose-THC preventive treatment on neuroinflammation and cognitive decline in 5xFAD mice
Nitzan K, Bentulila Z, Bregman-Yemini N, Ayalon N, David D et al.
Biol Sex Differ • 2026
Morris Water Maze
behavioralMouse5xFAD mouse model and C57B/Rcc
Objective: Learning ability and spatial memory evaluation using classical spatial learning assay
Materials & Equipment Checklist
4 items
Gather these items before starting the experiment. Check off items as you prepare.
Equipment3
Materials1
Not specified • Not specified
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Protocol Steps
1
Pool setup and temperature control
Set up round pool with spatial cues visible and maintain water temperature
Not specified
View evidence from paper
“spatial cues (such as a black cross on the white wall, etc.) were visible for the mice, and was allowed 60 s to find the platform. The water was kept at a temperature of 23 degrees Celsius”
2
Training phase - Days 1-3
Introduce mouse into pool at different starting points, allow 60 seconds to find immersed platform, record time and route
60 seconds maximum per trial, 4 trials per day for 3 consecutive days
View evidence from paper
“Each mouse swam four times per day for three consecutive days. The time and route required for the mouse to find the immersed platform was recorded”
3
Probe test - Day 4
Remove platform and allow mouse to swim, record time spent in platform zone
60 seconds
View evidence from paper
“On day 4 of the assay, the platform was removed, and the mouse was allowed to swim for 60 s ("probe test"). The time the mice spent in the platform zone (the location of the missing platform) was recorded”