Source Paper
Sex-dependent effects of ultra-low-dose-THC preventive treatment on neuroinflammation and cognitive decline in 5xFAD mice
Nitzan K, Bentulila Z, Bregman-Yemini N, Ayalon N, David D et al.
Biol Sex Differ • 2026
Morris Water Maze
Objective: Learning ability and spatial memory evaluation using classical spatial learning assay
This is a Morris Water Maze protocol using Mouse as the model organism. The procedure involves 3 procedural steps, 3 equipment items, 1 materials. Extracted from a 2026 paper published in Biol Sex Differ.
Model and subjects
Mouse • 5xFAD mouse model and C57B/Rcc • Male and female • 6 months at testing • 80
Study window
Estimated timing pending
Core workflow
Pool setup and temperature control • Training phase - Days 1-3 • Probe test - Day 4
Primary readouts
- Time required to find the immersed platform
- Route taken to find the platform
- Time spent in platform zone during probe test
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Pool setup and temperature control
Set up round pool with spatial cues visible and maintain water temperature
View evidence from paper
“spatial cues (such as a black cross on the white wall, etc.) were visible for the mice, and was allowed 60 s to find the platform. The water was kept at a temperature of 23 degrees Celsius”
Training phase - Days 1-3
Introduce mouse into pool at different starting points, allow 60 seconds to find immersed platform, record time and route
View evidence from paper
“Each mouse swam four times per day for three consecutive days. The time and route required for the mouse to find the immersed platform was recorded”
Probe test - Day 4
Remove platform and allow mouse to swim, record time spent in platform zone
View evidence from paper
“On day 4 of the assay, the platform was removed, and the mouse was allowed to swim for 60 s ("probe test"). The time the mice spent in the platform zone (the location of the missing platform) was recorded”