Morris Water Maze - Repeated-Reversal Version
Objective: To assess learning of new platform positions and cognitive flexibility in transgenic and control mice using a repeated-reversal version of the Morris water maze, and to evaluate whether environmental enrichment mitigates cognitive deficits
This is a Morris Water Maze - Repeated-Reversal Version protocol using mouse as the model organism. The procedure involves 3 procedural steps, 2 equipment items. Extracted from a 2005 paper published in Journal of Neuroscience.
Model and subjects
mouse • transgenic mice overexpressing amyloid precursor protein and/or presenilin-1 and nontransgenic controls • female • 2 months at start of housing condition, tested after 6 months of differential housing
Study window
Estimated timing pending
Core workflow
Housing Assignment • Cognitive Behavior Testing • Repeated-Reversal Task
Primary readouts
- Performance in radial water maze
- Performance in classic Morris water maze
- Performance in repeated-reversal Morris water maze
- Memory for platform location
Key equipment and reagents
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Protocol Steps
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Housing Assignment
Female transgenic mice overexpressing amyloid precursor protein and/or presenilin-1 and nontransgenic controls were placed into either enriched or standard cages
Note: Housing assignment began at 2 months of age
View evidence from paper
“Female transgenic mice overexpressing amyloid precursor protein and/or presenilin-1 and nontransgenic controls were placed into enriched or standard cages at 2 months of age”
Cognitive Behavior Testing
After 6 months of differential housing, mice were tested for cognitive behavior using radial water maze and Morris water maze tasks
Note: Testing included both classic and repeated-reversal versions of the Morris water maze
View evidence from paper
“tested for cognitive behavior after 6 months of differential housing. Enrichment significantly improved performance of all genotypes in the radial water maze and in the classic and repeated-reversal versions of the Morris water maze”
Repeated-Reversal Task
Mice learned successive new platform positions in the Morris water maze, with platform location changed between trials to assess cognitive flexibility and learning speed
Note: Performance measured by how quickly mice learned new platform positions
View evidence from paper
“learned new platform positions in the repeated-reversals task less quickly than their nontransgenic cagemates”