Morris Water Maze - Repeated-Reversal Version
Objective: To assess learning of new platform positions and cognitive flexibility in transgenic and control mice using a repeated-reversal version of the Morris water maze, and to evaluate whether environmental enrichment mitigates cognitive deficits
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Protocol Steps
Housing Assignment
Female transgenic mice overexpressing amyloid precursor protein and/or presenilin-1 and nontransgenic controls were placed into either enriched or standard cages
Note: Housing assignment began at 2 months of age
View evidence from paper
“Female transgenic mice overexpressing amyloid precursor protein and/or presenilin-1 and nontransgenic controls were placed into enriched or standard cages at 2 months of age”
Cognitive Behavior Testing
After 6 months of differential housing, mice were tested for cognitive behavior using radial water maze and Morris water maze tasks
Note: Testing included both classic and repeated-reversal versions of the Morris water maze
View evidence from paper
“tested for cognitive behavior after 6 months of differential housing. Enrichment significantly improved performance of all genotypes in the radial water maze and in the classic and repeated-reversal versions of the Morris water maze”
Repeated-Reversal Task
Mice learned successive new platform positions in the Morris water maze, with platform location changed between trials to assess cognitive flexibility and learning speed
Note: Performance measured by how quickly mice learned new platform positions
View evidence from paper
“learned new platform positions in the repeated-reversals task less quickly than their nontransgenic cagemates”