Source Paper
Lipopolysaccharide induces delayed FosB/DeltaFosB immunostaining within the mouse extended amygdala, hippocampus and hypothalamus, that parallel the expression of depressive-like behavior
François Frenois, Maïté Moreau, Jason O’Connor, Marc Lawson, Charlotte Micon et al.
Psychoneuroendocrinology • 2007
Motor Activity Test
Objective: Measurement of mouse motor activity in a new cage at 6 and 24 hours post-LPS treatment to assess sickness behavior and dissociate it from depressive-like behavior
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
LPS Administration
Administer LPS via intraperitoneal injection at dose of 830 μg/kg
Note: Intraperitoneal route of administration
View evidence from paper
“lipopolysaccharide (LPS, 830 μg/kg, intraperitoneal)”
Motor Activity Measurement at 6 Hours
Place mice in a new cage and measure motor activity at 6 hours post-LPS treatment
Note: At this timepoint, sickness behavior is expected to be maximal
View evidence from paper
“LPS-induced depressive-like behavior was dissociated from LPS-induced sickness by testing mice either at 6 h (at which time sickness was expected to be maximal)”
Motor Activity Measurement at 24 Hours
Place mice in a new cage and measure motor activity at 24 hours post-LPS treatment
Note: At this timepoint, sickness is expected to be minimal and not bias measurement of depressive-like behavior
View evidence from paper
“or at 24 h post-LPS (at which time sickness was expected to be minimal and not to bias the measurement of depressive-like behavior)”