Source Paper
Lipopolysaccharide induces delayed FosB/DeltaFosB immunostaining within the mouse extended amygdala, hippocampus and hypothalamus, that parallel the expression of depressive-like behavior
François Frenois, Maïté Moreau, Jason O’Connor, Marc Lawson, Charlotte Micon et al.
Psychoneuroendocrinology • 2007
Motor Activity Test
Objective: Measurement of mouse motor activity in a new cage at 6 and 24 hours post-LPS treatment to assess sickness behavior and dissociate it from depressive-like behavior
This is a Motor Activity Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 2 materials. Extracted from a 2007 paper published in Psychoneuroendocrinology.
Model and subjects
mouse • Not specified • unknown • Not specified • Not specified
Study window
~60 hours hands-on
Core workflow
LPS Administration • Motor Activity Measurement at 6 Hours • Motor Activity Measurement at 24 Hours
Primary readouts
- Motor activity in new cage at 6 hours post-LPS
- Motor activity in new cage at 24 hours post-LPS
- Comparison of motor activity between LPS-treated and saline control groups
Key equipment and reagents
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Protocol Steps
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LPS Administration
Administer LPS via intraperitoneal injection at dose of 830 µg/kg
Note: Intraperitoneal route of administration
View evidence from paper
“lipopolysaccharide (LPS, 830 µg/kg, intraperitoneal)”
Motor Activity Measurement at 6 Hours
Place mice in a new cage and measure motor activity at 6 hours post-LPS treatment
Note: At this timepoint, sickness behavior is expected to be maximal
View evidence from paper
“LPS-induced depressive-like behavior was dissociated from LPS-induced sickness by testing mice either at 6 h (at which time sickness was expected to be maximal)”
Motor Activity Measurement at 24 Hours
Place mice in a new cage and measure motor activity at 24 hours post-LPS treatment
Note: At this timepoint, sickness is expected to be minimal and not bias measurement of depressive-like behavior
View evidence from paper
“or at 24 h post-LPS (at which time sickness was expected to be minimal and not to bias the measurement of depressive-like behavior)”