Source Paper
NAD⁺ Reduction in Glutamatergic Neurons Induces Lipid Catabolism and Neuroinflammation in the Brain via SARM1
Niou Z, Yang S, Enriquez A, Espinas NA, Sri A et al.
Adv Sci (Weinh) • 2025
Motor Behavioral Assessment
Objective: Assessment of motor behavioral deficits in mice with neuronal NMNAT2 loss
This is a Motor Behavioral Assessment protocol using Mouse as the model organism. The procedure involves 2 procedural steps. Extracted from a 2025 paper published in Adv Sci (Weinh).
Model and subjects
Mouse • NMNAT2 conditional knockout (cKO) and control littermates • Both male and female • P16-P21 • 12
Study window
Estimated timing pending
Core workflow
Generate experimental mice • Assess motor behavioral deficits
Primary readouts
- Motor behavioral deficits
- Neurodegenerative phenotypes
Key equipment and reagents
Verified items
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Direct vendor links
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Protocol Steps
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Generate experimental mice
Cross NMNAT2 conditional allele mice (NMNAT2 f/f) with Nex-Cre transgenic mice to create cortical glutamatergic neuron-specific NMNAT2 knockout (cKO) mice
View evidence from paper
“The cKO mice were generated by crossing NMNAT2 conditional allele mice (NMNAT2 f/f) with a Nex‐Cre transgenic mice”
Assess motor behavioral deficits
Evaluate motor behaviors in P16-P21 NMNAT2 cKO mice compared to littermate controls
View evidence from paper
“pronounced neurodegenerative phenotypes and motor behavioral deficits”