Source Paper
NAD⁺ Reduction in Glutamatergic Neurons Induces Lipid Catabolism and Neuroinflammation in the Brain via SARM1
Niou Z, Yang S, Enriquez A, Espinas NA, Sri A et al.
Adv Sci (Weinh) • 2025
Motor Behavioral Assessment
behavioralMouseNMNAT2 conditional knockout (cKO) and control littermates
Objective: Assessment of motor behavioral deficits in mice with neuronal NMNAT2 loss
Protocol Steps
1
Generate experimental mice
Cross NMNAT2 conditional allele mice (NMNAT2 f/f) with Nex-Cre transgenic mice to create cortical glutamatergic neuron-specific NMNAT2 knockout (cKO) mice
Not specified
View evidence from paper
“The cKO mice were generated by crossing NMNAT2 conditional allele mice (NMNAT2 f/f) with a Nex‐Cre transgenic mice”
2
Assess motor behavioral deficits
Evaluate motor behaviors in P16-P21 NMNAT2 cKO mice compared to littermate controls
Not specified
View evidence from paper
“pronounced neurodegenerative phenotypes and motor behavioral deficits”