Motor Coordination Assessment
Objective: Assessment of motor coordination deficits in stroke mice over 28 days post-ischemia following MSC-EV or MSC treatment
This is a Motor Coordination Assessment protocol using mouse as the model organism. The procedure involves 8 procedural steps, 1 equipment items. Extracted from a 2015 paper published in Stem Cells Translational Medicine.
Model and subjects
mouse • C57BL/6 • unknown • Not specified • Not specified
Study window
~4 week study window
Core workflow
Induce focal cerebral ischemia • Administer MSC-EVs treatment • Administer MSCs treatment
Primary readouts
- Motor coordination deficits
- Histological brain injury
- Immune responses in peripheral blood (B-cell, natural killer cell, T-cell lymphopenia)
- Cerebral immune cell infiltration
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Induce focal cerebral ischemia
Focal cerebral ischemia was induced in C57BL/6 mice
Note: Baseline for all experimental groups
View evidence from paper
“mesenchymal stem cell (MSC)-derived EVs were compared with those of MSCs i.v. delivered 1, 3, and 5 days or 1 day after focal cerebral ischemia in mice”
Administer MSC-EVs treatment
MSC-derived extracellular vesicles administered intravenously at 1, 3, and 5 days post-ischemia
Note: Multiple timepoint administration protocol
View evidence from paper
“mesenchymal stem cell (MSC)-derived EVs were compared with those of MSCs i.v. delivered 1, 3, and 5 days or 1 day after focal cerebral ischemia in mice”
Administer MSCs treatment
Mesenchymal stem cells administered intravenously at 1 day post-ischemia
Note: Single timepoint administration for MSC group
View evidence from paper
“mesenchymal stem cell (MSC)-derived EVs were compared with those of MSCs i.v. delivered 1, 3, and 5 days or 1 day after focal cerebral ischemia in mice”
Assess motor coordination deficits
Motor coordination deficits measured throughout observation period
Note: Longitudinal assessment over 28-day period
View evidence from paper
“For as long as 28 days after stroke, motor coordination deficits, histological brain injury, immune responses in the peripheral blood and brain, and cerebral angiogenesis and neurogenesis were analyzed.”
Analyze immune responses in peripheral blood
Immune responses measured in peripheral blood including B-cell, natural killer cell, and T-cell lymphopenia assessment
Note: Postischemic immunosuppression evaluated
View evidence from paper
“postischemic immunosuppression (i.e., B-cell, natural killer cell, and T-cell lymphopenia) was attenuated in the peripheral blood at 6 days after ischemia”
Analyze immune responses in brain
Cerebral immune cell infiltration assessed
Note: Not affected by MSC-EVs according to results
View evidence from paper
“Although cerebral immune cell infiltration was not affected by MSC-EVs, postischemic immunosuppression was attenuated in the peripheral blood”
Assess histological brain injury
Histological analysis of brain injury performed
Note: Part of comprehensive outcome assessment
View evidence from paper
“For as long as 28 days after stroke, motor coordination deficits, histological brain injury, immune responses in the peripheral blood and brain, and cerebral angiogenesis and neurogenesis were analyzed.”
Assess cerebral angiogenesis and neurogenesis
Cerebral angiogenesis and neurogenesis evaluated as markers of brain remodeling
Note: Enhanced angioneurogenesis observed in EV-treated mice
View evidence from paper
“Improved neurological impairment and long-term neuroprotection associated with enhanced angioneurogenesis were noticed in stroke mice receiving EVs”