Source Paper
Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4/NFκB/NLRP3 signaling pathway in cerebral ischemic mice
Lu Luo, Meixi Liu, Yunhui Fan, Jingjun Zhang, Li Liu et al.
Journal of Neuroinflammation • 2022
Motor Function Assessment
Objective: Evaluate motor function in MCAO/r injury mice following iTBS treatment with or without microglial depletion, and explore morphological and molecular alterations associated with neuronal pyroptosis and microglial polarization
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Equipment1
Not specified • Not specified • Not specified • Not specified
Materials1
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Protocol Steps
MCAO/r injury induction
Induce middle cerebral artery occlusion/reperfusion injury in mice
Note: Baseline condition for all experimental groups
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“middle cerebral artery occlusion/reperfusion (MCAO/r) injury mice”
Microglial depletion treatment
Treat mice with CSF1R inhibitor to deplete microglia in designated groups
Note: Applied to one experimental group only
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“depletion of microglia by colony-stimulating factor 1 receptor (CSF1R) inhibitor treatment”
iTBS treatment
Apply intermittent theta-burst rTMS treatment to mice
Note: Applied to all experimental groups in early phase post-injury
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“1-week intermittent theta-burst rTMS (iTBS) treatment in the early phase”
Motor function evaluation
Evaluate motor function in treated mice
Note: Primary outcome measure
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“We evaluated the motor function in middle cerebral artery occlusion/reperfusion (MCAO/r) injury mice”
Nissl staining
Perform Nissl staining for morphological analysis
Note: Histological staining procedure
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“Nissl, EdU, TTC, TUNEL staining”
EdU staining
Perform EdU staining for cell proliferation analysis
Note: Histological staining procedure
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“Nissl, EdU, TTC, TUNEL staining”
TTC staining
Perform TTC staining for infarct visualization
Note: Histological staining procedure
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“Nissl, EdU, TTC, TUNEL staining”
TUNEL staining
Perform TUNEL staining for apoptosis detection
Note: Histological staining procedure
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“Nissl, EdU, TTC, TUNEL staining”
Electron microscopy analysis
Perform electron microscopy for ultrastructural morphological analysis
Note: Ultrastructural analysis
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“electron microscopy”
Multiplex cytokine bioassays
Perform multiplex cytokine bioassays to measure inflammatory markers
Note: Molecular analysis of cytokine profiles
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“multiplex cytokine bioassays”
Western blot assays
Perform western blot assays for protein analysis
Note: Molecular analysis of protein expression
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“western blot assays”
Immunofluorescence staining
Perform immunofluorescence staining for cellular localization analysis
Note: Immunological analysis
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“immunofluorescence staining”
RNA sequencing
Perform RNA sequencing for gene expression analysis
Note: Genomic analysis
View evidence from paper
“RNA sequencing”