Experiments/Myocardial Infarction by Coronary Ligation

Source Paper

The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions

Matthias Nahrendorf, Filip K. Swirski, Elena Aikawa, Lars Stangenberg, Thomas Wurdinger et al.

The Journal of Experimental Medicine • 2007

DOI PMC

Myocardial Infarction by Coronary Ligation

behavioralmouseC57BL/6, apoE −/−, CCR2 −/−, CX3CR1 −/−

Objective: Induce myocardial infarction in mice through permanent ligation of the left coronary artery to study cardiac pathology and immune responses

Materials & Equipment Checklist

9 items2 from ConductScience

Gather these items before starting the experiment. Check off items as you prepare.

Equipment3

Intubation equipment

not specified • not specified • not specified • not specified

Amazon

Ventilator

not specified • not specified • not specified • not specified

Amazon

Surgical microscope or visualization equipment

not specified • not specified • not specified • not specified

Amazon

Materials6

Western diet

Harlan Teklad, Inc. • not specified • not specified • not specified

Amazon

Monofilament nylon suture

not specified • 8-0 gauge • not specified • not specified

$400

Sutures

not specified • not specified • not specified • not specified

$400

Superglue

not specified • not specified • not specified • not specified

Amazon

Isoflurane

not specified • not specified • not specified • not specified

Oxygen

not specified • not specified • not specified • not specified

Amazon

2 items available from ConductScience

Estimated: $800.00

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Protocol Steps

Anesthesia induction and intubation

Mice were intubated and ventilated with isoflurane supplemented with oxygen

not specifiednot specified

Note: Anesthetic concentration: 1-2% vol/vol isoflurane with 2 liters O2

View evidence from paper

“Mice were intubated and ventilated with isoflurane supplemented with oxygen (isoflurane 1–2% vol/vol + 2 liters O2)”

Surgical site preparation

The chest wall was shaved in preparation for thoracotomy

not specifiednot specified

Note: Shaving prepares the surgical site for incision

View evidence from paper

“The chest wall was shaved and a thoracotomy was performed in the fourth left intercostal space”

Thoracotomy

A thoracotomy was performed in the fourth left intercostal space to access the heart

not specifiednot specified

Note: Incision location: fourth left intercostal space

View evidence from paper

“a thoracotomy was performed in the fourth left intercostal space”

Visualization of left ventricle and coronary artery

The left ventricle was visualized to identify the left coronary artery

not specifiednot specified

Note: Visualization allows identification of the coronary artery at its emergence from under the left atrium

View evidence from paper

“The left ventricle was visualized, and the left coronary artery was permanently ligated with a monofilament nylon 8–0 suture”

Permanent ligation of left coronary artery

The left coronary artery was permanently ligated with a monofilament nylon 8-0 suture at the site of its emergence from under the left atrium

not specifiednot specified

Silicone Coated MCAO Monofilament Suture

Matches: Monofilament nylon suture

$400View

Note: Permanent ligation induces myocardial infarction. Suture type: monofilament nylon 8-0 gauge

View evidence from paper

“the left coronary artery was permanently ligated with a monofilament nylon 8–0 suture at the site of its emergence from under the left atrium”

Closure of thoracotomy

The thoracotomy was closed with sutures and superglue

not specifiednot specified

Silicone Coated MCAO Monofilament Suture

Matches: Sutures

$400View

Note: Combination of sutures and superglue used for wound closure

View evidence from paper

“The thoracotomy was closed with sutures and superglue”