Source Paper
The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions
Matthias Nahrendorf, Filip K. Swirski, Elena Aikawa, Lars Stangenberg, Thomas Wurdinger et al.
The Journal of Experimental Medicine • 2007
Myocardial Infarction by Coronary Ligation
Objective: Induce myocardial infarction in mice through permanent ligation of the left coronary artery to study cardiac pathology and immune responses
This is a Myocardial Infarction by Coronary Ligation protocol using mouse as the model organism. The procedure involves 6 procedural steps, 3 equipment items, 6 materials. Extracted from a 2007 paper published in The Journal of Experimental Medicine.
Model and subjects
mouse • C57BL/6, apoE −/−, CCR2 −/−, CX3CR1 −/− • female • 6-8 weeks (except apoE −/− mice at 1 year old) • not specified • 160
Study window
Estimated timing pending
Core workflow
Anesthesia induction and intubation • Surgical site preparation • Thoracotomy
Primary readouts
- Myocardial infarction induction (confirmed by coronary artery ligation)
- Cardiac pathology and damage
- Immune cell infiltration and response
Key equipment and reagents
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Protocol Steps
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Anesthesia induction and intubation
Mice were intubated and ventilated with isoflurane supplemented with oxygen
Note: Anesthetic concentration: 1-2% vol/vol isoflurane with 2 liters O2
View evidence from paper
“Mice were intubated and ventilated with isoflurane supplemented with oxygen (isoflurane 1–2% vol/vol + 2 liters O2)”
Surgical site preparation
The chest wall was shaved in preparation for thoracotomy
Note: Shaving prepares the surgical site for incision
View evidence from paper
“The chest wall was shaved and a thoracotomy was performed in the fourth left intercostal space”
Thoracotomy
A thoracotomy was performed in the fourth left intercostal space to access the heart
Note: Incision location: fourth left intercostal space
View evidence from paper
“a thoracotomy was performed in the fourth left intercostal space”
Visualization of left ventricle and coronary artery
The left ventricle was visualized to identify the left coronary artery
Note: Visualization allows identification of the coronary artery at its emergence from under the left atrium
View evidence from paper
“The left ventricle was visualized, and the left coronary artery was permanently ligated with a monofilament nylon 8–0 suture”
Permanent ligation of left coronary artery
The left coronary artery was permanently ligated with a monofilament nylon 8-0 suture at the site of its emergence from under the left atrium
Note: Permanent ligation induces myocardial infarction. Suture type: monofilament nylon 8-0 gauge
View evidence from paper
“the left coronary artery was permanently ligated with a monofilament nylon 8–0 suture at the site of its emergence from under the left atrium”
Closure of thoracotomy
The thoracotomy was closed with sutures and superglue
Note: Combination of sutures and superglue used for wound closure
View evidence from paper
“The thoracotomy was closed with sutures and superglue”