Neurological Recovery Assessment
Objective: Assess whether a delay in decompression influences the extent of ischemia-reperfusion injury and alters the trajectory of neurological outcome in degenerative cervical myelopathy using a DCM mouse model
This is a Neurological Recovery Assessment protocol using mouse as the model organism. The procedure involves 7 procedural steps. Extracted from a 2017 paper published in JCI Insight.
Model and subjects
mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Early Decompression Group • Delayed Decompression Group • Measure Inflammatory Cytokine Levels
Primary readouts
- Inflammatory cytokine levels in spinal cord
- Neurological recovery trajectory
- Hyperalgesia assessment
- Peripheral monocytic inflammatory response
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Early Decompression Group
Perform surgical decompression early in the disease course
Note: Early decompression led to resolution of reperfusion-mediated inflammation, neurological improvement, and reduced hyperalgesia
View evidence from paper
“early decompression led to resolution of reperfusion-mediated inflammation, neurological improvement, and reduced hyperalgesia”
Delayed Decompression Group
Perform surgical decompression after a delay in the disease course
Note: Delayed decompression was associated with exacerbated reperfusion injury, astrogliosis, and poorer neurological recovery
View evidence from paper
“Delayed decompression was associated with exacerbated reperfusion injury, astrogliosis, and poorer neurological recovery”
Measure Inflammatory Cytokine Levels
Assess levels of inflammatory cytokines within the spinal cord following decompression
Note: Decompression acutely led to a 1.5- to 2-fold increase in levels of inflammatory cytokines within the spinal cord
View evidence from paper
“decompression acutely led to a 1.5- to 2-fold increase in levels of inflammatory cytokines within the spinal cord”
Assess Neurological Recovery
Evaluate neurological improvement in early versus delayed decompression groups
Note: Early decompression associated with neurological improvement; delayed decompression with poorer neurological recovery
View evidence from paper
“early decompression led to resolution of reperfusion-mediated inflammation, neurological improvement, and reduced hyperalgesia”
Evaluate Hyperalgesia
Assess pain sensitivity and hyperalgesia in both treatment groups
Note: Early decompression resulted in reduced hyperalgesia
View evidence from paper
“early decompression led to resolution of reperfusion-mediated inflammation, neurological improvement, and reduced hyperalgesia”
Measure Peripheral Monocytic Inflammatory Response
Assess peripheral monocytic inflammatory response in both groups
Note: Delayed decompression associated with exacerbated peripheral monocytic inflammatory response (P < 0.01 and 0.001)
View evidence from paper
“delayed decompression was associated with prolonged elevation of inflammatory cytokines and an exacerbated peripheral monocytic inflammatory response ( P < 0.01 and 0.001)”
Assess Astrogliosis and Microglia Activation
Evaluate astrogliosis and microglia activation in spinal cord tissue
Note: Delayed decompression associated with exacerbated astrogliosis; delayed surgical decompression associated with ongoing microglia activation
View evidence from paper
“Delayed decompression was associated with exacerbated reperfusion injury, astrogliosis, and poorer neurological recovery”