Source Paper
Synaptic changes contribute to persistent extra-motor behaviour deficits in amyotrophic lateral sclerosis
Luan W, San Gil R, Madrid San Martin L, Cao MC, Vassallu F et al.
Acta Neuropathol Commun • 2025
Neurological Score
behavioralMouserNLS8 mice (C57BL/6JAusb background)
Objective: Assessment of neurological deficits through observation of hindlimb positioning during tail suspension
Materials & Equipment Checklist
1 item
Gather these items before starting the experiment. Check off items as you prepare.
Materials1
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Protocol Steps
1
Housing conditions
House mice in controlled environmental conditions with 12-hour light/dark cycle
View evidence from paper
“housed in temperature- and humidity-controlled conditions (21 ± 1 °C, 55 ± 5%)”
2
Diet switch
Switch mice from doxycycline-containing chow to normal chow at approximately ten weeks of age
View evidence from paper
“switched to normal chow (SF00-100, Specialty Feeds, Australia) to induce expression”
3
Tail suspension
Suspend mice by the tail for greater than 5 seconds
View evidence from paper
“by tail suspension for > 5 s as previously described”
4
Neurological assessment
Observe and score hindlimb positioning on a scale of 0 to 4, looking for collapsing splay or clasping behaviors
View evidence from paper
“assessed for observation of collapsing splay or clasping of hindlimbs on a scale of 0 to 4”