Source Paper
Synaptic changes contribute to persistent extra-motor behaviour deficits in amyotrophic lateral sclerosis
Luan W, San Gil R, Madrid San Martin L, Cao MC, Vassallu F et al.
Acta Neuropathol Commun • 2025
Neurological Score
Objective: Assessment of neurological deficits through observation of hindlimb positioning during tail suspension
This is a Neurological Score protocol using Mouse as the model organism. The procedure involves 4 procedural steps, 1 materials. Extracted from a 2025 paper published in Acta Neuropathol Commun.
Model and subjects
Mouse • rNLS8 mice (C57BL/6JAusb background) • Male and female • Approximately ten weeks of age at test initiation • Variable per experiment (see individual figure legends)
Study window
Estimated timing pending
Core workflow
Housing conditions • Diet switch • Tail suspension
Primary readouts
- Neurological score (0-4 scale) based on hindlimb positioning during tail suspension
Key equipment and reagents
Verified items
0
Direct vendor links
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Protocol Steps
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Housing conditions
House mice in controlled environmental conditions with 12-hour light/dark cycle
View evidence from paper
“housed in temperature- and humidity-controlled conditions (21 ± 1 °C, 55 ± 5%)”
Diet switch
Switch mice from doxycycline-containing chow to normal chow at approximately ten weeks of age
View evidence from paper
“switched to normal chow (SF00-100, Specialty Feeds, Australia) to induce expression”
Tail suspension
Suspend mice by the tail for greater than 5 seconds
View evidence from paper
“by tail suspension for > 5 s as previously described”
Neurological assessment
Observe and score hindlimb positioning on a scale of 0 to 4, looking for collapsing splay or clasping behaviors
View evidence from paper
“assessed for observation of collapsing splay or clasping of hindlimbs on a scale of 0 to 4”