Source Paper
Morin improves postoperative cognitive dysfunction by mediating the miR-138-5p/SIRT1 axis to regulate ferroptosis
Yang Y, Hu W, Wu Z, Chen M, Li H et al.
Sci Rep • 2026
Novel Location Recognition (NLR)
Objective: To assess spatial memory and recognition of novel object locations using behavioral testing
This is a Novel Location Recognition (NLR) protocol using Mouse as the model organism. The procedure involves 8 procedural steps, 1 equipment items, 3 materials. Extracted from a 2026 paper published in Sci Rep.
Model and subjects
Mouse • C57BL/6J • Not specified • 8 weeks old • 6
Study window
~1 hour study window | ~10 minutes hands-on
Core workflow
Acclimatization • Clean equipment • Acquisition trial
Primary readouts
- Time spent exploring object at new location (A')
- Time spent exploring object at old location (B)
- Percentage of exploration time calculated as T(Novel)/T(Total) × 100%
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Acclimatization
Place mice in testing room for adaptation, then habituate to empty box
View evidence from paper
“mice were acclimated to the testing room for 1 h and then placed into the empty box to habituate for 10 min”
Clean equipment
Clean box and objects with 75% ethanol between sessions
View evidence from paper
“The box and objects were cleaned with 75% ethanol after each session to eliminate any residual odor”
Acquisition trial
Place mice in arena with two identical objects in opposite corners for 10-minute exploration
View evidence from paper
“mice were placed in an arena containing two identical objects (A and B) placed in symmetrically opposite corners for a 10-minute acquisition trial”
Record exploration time
Record time spent exploring each object during acquisition
View evidence from paper
“The time spent exploring each object (A and B) was recorded”
Clean after acquisition
Clean box and objects with 75% ethanol after acquisition trial
View evidence from paper
“Afterward, the box and objects were cleaned with 75% ethanol”
NLR test setup
Move object A to new location (A') 1 hour after training
View evidence from paper
“For NLR task, 1 h after training, object A was moved to a new location (A')”
NLR test trial
Allow mice to explore for 10 minutes and record exploration times
View evidence from paper
“Mice were reintroduced to the box and allowed to explore for 10 min. The time spent exploring the object at the new location (A') and the object at the old location (B) was recorded”
Final cleaning
Clean box and objects with 75% ethanol after testing
View evidence from paper
“The box and objects were cleaned with 75% ethanol after testing”