Source Paper
Morin improves postoperative cognitive dysfunction by mediating the miR-138-5p/SIRT1 axis to regulate ferroptosis
Yang Y, Hu W, Wu Z, Chen M, Li H et al.
Sci Rep • 2026
Novel Location Recognition (NLR)
Objective: To assess spatial memory and recognition of novel object locations using behavioral testing
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Protocol Steps
Acclimatization
Place mice in testing room for adaptation, then habituate to empty box
View evidence from paper
“mice were acclimated to the testing room for 1 h and then placed into the empty box to habituate for 10 min”
Clean equipment
Clean box and objects with 75% ethanol between sessions
View evidence from paper
“The box and objects were cleaned with 75% ethanol after each session to eliminate any residual odor”
Acquisition trial
Place mice in arena with two identical objects in opposite corners for 10-minute exploration
View evidence from paper
“mice were placed in an arena containing two identical objects (A and B) placed in symmetrically opposite corners for a 10-minute acquisition trial”
Record exploration time
Record time spent exploring each object during acquisition
View evidence from paper
“The time spent exploring each object (A and B) was recorded”
Clean after acquisition
Clean box and objects with 75% ethanol after acquisition trial
View evidence from paper
“Afterward, the box and objects were cleaned with 75% ethanol”
NLR test setup
Move object A to new location (A') 1 hour after training
View evidence from paper
“For NLR task, 1 h after training, object A was moved to a new location (A')”
NLR test trial
Allow mice to explore for 10 minutes and record exploration times
View evidence from paper
“Mice were reintroduced to the box and allowed to explore for 10 min. The time spent exploring the object at the new location (A') and the object at the old location (B) was recorded”
Final cleaning
Clean box and objects with 75% ethanol after testing
View evidence from paper
“The box and objects were cleaned with 75% ethanol after testing”