Source Paper
Morin improves postoperative cognitive dysfunction by mediating the miR-138-5p/SIRT1 axis to regulate ferroptosis
Yang Y, Hu W, Wu Z, Chen M, Li H et al.
Sci Rep • 2026
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Acclimatization to testing room
Allow mice to acclimate to the testing room for 1 hour
View evidence from paper
“mice were acclimated to the testing room for 1 h”
Habituation to empty box
Place mice into empty box for 10 minutes of habituation
View evidence from paper
“then placed into the empty box to habituate for 10 min”
Clean arena and objects
Clean box and objects with 75% ethanol to eliminate residual odors
View evidence from paper
“The box and objects were cleaned with 75% ethanol after each session to eliminate any residual odor”
Initial acquisition trial
Place mice in arena with two identical objects (A and B) in opposite corners for 10-minute exploration
View evidence from paper
“mice were placed in an arena containing two identical objects (A and B) placed in symmetrically opposite corners for a 10-minute acquisition trial”
Record exploration time
Record time spent exploring each object (A and B) during acquisition
View evidence from paper
“The time spent exploring each object (A and B) was recorded”
Clean arena after acquisition
Clean the box and objects with 75% ethanol after acquisition trial
View evidence from paper
“Afterward, the box and objects were cleaned with 75% ethanol”
Novel Location Recognition (NLR) phase
One hour after training, move object A to new location (A') and test for 10 minutes
View evidence from paper
“For NLR task, 1 h after training, object A was moved to a new location (A'). Mice were reintroduced to the box and allowed to explore for 10 min”
Clean arena after NLR
Clean the box and objects with 75% ethanol after NLR testing
View evidence from paper
“The box and objects were cleaned with 75% ethanol after testing”
Novel Object Recognition (NOR) phase
One hour after NLR, replace object B with novel object C and test for 10 minutes
View evidence from paper
“For NOR task, 1 h after the NLR, object B was replaced with a novel object (C). Mice were tested for 10 min”
Record NOR exploration times
Record time spent exploring novel object C and familiar object at location A'
View evidence from paper
“the time spent exploring the novel object (C) and the familiar object at the location (A') was recorded”