Novel Object Recognition
Objective: Measure cognitive function and memory by assessing preference for novel objects in a chronic social defeat stress depression model
This is a Novel Object Recognition protocol using mouse as the model organism. The procedure involves 6 procedural steps, 1 equipment items, 4 materials. Extracted from a 2022 paper published in Journal of Neuroinflammation.
Model and subjects
mouse • Not specified • unknown • Not specified • Not specified
Study window
~30 minute study window | ~1 hours hands-on
Core workflow
Chronic Social Defeat Stress (CSDS) Model Implementation • Inhibitor Administration • EDA Treatment Administration
Primary readouts
- Preference for novel objects (primary NOR measure)
- Depressive behaviors
- Anxiety-like behaviors
- Cognitive function
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Chronic Social Defeat Stress (CSDS) Model Implementation
Establish depression model through chronic social defeat stress protocol
Note: This is the baseline condition for all subsequent behavioral tests
View evidence from paper
“A chronic social defeat stress (CSDS) depression model was performed to explore whether EDA could produce antidepressant effects”
Inhibitor Administration
Inject EX527 (Sirt1 inhibitor) and ML385 (Nrf2 inhibitor) intraperitoneally 30 minutes before EDA injection
Note: Administered daily
View evidence from paper
“EX527, a Sirt1 inhibitor and ML385, a Nrf2 inhibitor were injected intraperitoneally 30 min before EDA injection daily”
EDA Treatment Administration
Administer EDA compound following inhibitor injection
Note: Administered after 30 minute interval from inhibitor injection
View evidence from paper
“EX527, a Sirt1 inhibitor and ML385, a Nrf2 inhibitor were injected intraperitoneally 30 min before EDA injection daily”
Novel Object Recognition Test
Conduct NOR test to assess cognitive function and memory by measuring preference for novel objects
Note: Part of comprehensive behavioral testing battery
View evidence from paper
“Behaviors tests were carried out to examine depressive, anxiety-like and cognitive behaviors including social interaction (SI) test, sucrose preference test (SPT), open field test (OFT), elevated plus maze (EPM), novel object recognition (NOR)”
AAV Vector Infusion for Gpx4 Knockdown
Perform knockdown experiments using adeno-associated virus vector containing miRNAi (Gpx4)–EGFP to determine effects of Gpx4 on CSDS mice with EDA treatment
Note: Conducted to assess role of Gpx4 in antidepressant effects
View evidence from paper
“Knockdown experiments were performed to determine the effects of Gpx4 on CSDS mice with EDA treatment by an adeno-associated virus (AAV) vector containing miRNAi (Gpx4)–EGFP infusion”
Tissue Collection
Collect hippocampal and medial prefrontal cortex (mPFC) tissues for molecular and histological analysis
Note: Tissues used for multiple downstream analyses
View evidence from paper
“Hippocampal and medial prefrontal cortex (mPFC) tissues were collected for Nissl staining, immunofluorescence, targeted energy metabolomics analysis”