Source Paper
Tui Na Acupressure Modulates Treg Immunosuppression via FoxP3/mTORC1 Signalling in ALS Mice
Han J, Xu X, Zhao Y, Xiao Y, Huang F et al.
Immunology • 2025
Novel Object Recognition Test
Objective: To assess cognitive memory function by measuring time spent exploring novel vs familiar objects
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Equipment1
not specified • not specified
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Protocol Steps
Familiarization phase
Place each mouse in the testing chamber with two identical objects (blocks of the same height and shape) for exploration
View evidence from paper
“placed in a 50 cm × 50 cm testing chamber with two identical objects”
Inter-trial interval
Remove mouse from testing chamber and wait between familiarization and test phases
View evidence from paper
“After 24 h, one object was replaced with a novel one”
Test phase
Replace one familiar object with a novel object and place mouse back in chamber
View evidence from paper
“one object was replaced with a novel one”
Data collection
Record the time spent exploring the novel object during the test phase
View evidence from paper
“The time spent exploring the new object (in seconds) was recorded”