Source Paper
Tui Na Acupressure Modulates Treg Immunosuppression via FoxP3/mTORC1 Signalling in ALS Mice
Han J, Xu X, Zhao Y, Xiao Y, Huang F et al.
Immunology • 2025
Novel Object Recognition Test
Objective: To assess cognitive memory function by measuring time spent exploring novel vs familiar objects
This is a Novel Object Recognition Test protocol using mice as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 2 materials. Extracted from a 2025 paper published in Immunology.
Model and subjects
mice • B6SJL‐TgN(SOD1 G93A )1Gur/J transgenic mice • not specified • 11, 14, 16, 18, and 20 weeks • 60
Study window
Estimated timing pending
Core workflow
Familiarization phase • Inter-trial interval • Test phase
Primary readouts
- Time spent exploring novel object (seconds)
- Cognitive memory status (intact if >65% exploration time on novel object)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Familiarization phase
Place each mouse in the testing chamber with two identical objects (blocks of the same height and shape) for exploration
View evidence from paper
“placed in a 50 cm × 50 cm testing chamber with two identical objects”
Inter-trial interval
Remove mouse from testing chamber and wait between familiarization and test phases
View evidence from paper
“After 24 h, one object was replaced with a novel one”
Test phase
Replace one familiar object with a novel object and place mouse back in chamber
View evidence from paper
“one object was replaced with a novel one”
Data collection
Record the time spent exploring the novel object during the test phase
View evidence from paper
“The time spent exploring the new object (in seconds) was recorded”